Summary
Mitochondrial DNA from the petite negative yeast Hansenula saturnus has been isolated and sized by digestion with restriction enzymes. The size of the mitochondrial genome is approximately 47 kb. The gene for subunit II of cytochrome oxidase was localized in the genome by Southern blotting using a [32P]-labeled probe containing the subunit II gene of the yeast Saccharomyces cerevisiae. The probe hybridized to a 1.7 kb HindIII-BamHI fragment under stringent conditions (65°C), indicating a high degree of homology between the S. cerevisiae and H. saturnus mitochondrial DNA fragments. The 1.7 kb fragment from H. saturnus was cloned into pBR322 and physically mapped. The map was used to obtain the nucleotide sequence of the subunit II gene (Lawson and Deters presented in the accompanying paper).
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References
Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717
Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AL, Drouin J, Eperson IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Barrell BG, Anderson S, Bankier AT, de Bruijn MHC, Chen E, Coulson AR, Drouin J, Eperson EC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1980) Proc Natl Acad Sci USA 77:3164–3166
Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Cabral F, Solioz M, Rudin Y, Schatz G, Clavilier L, Slonimski PP (1978) J Biol Chem 253:297–304
Clark-Walker GD, Sriprakash KS (1983) EMBO J 2:1465–1472
Cohen SN, Chang ACY, Hsu L (1972) Proc Natl Acad Sci USA 69:2110–2114
Coruzzi G, Tzagoloff A (1979) J Biol Chem 254:9324–9330
Deters DW, Muller U, Homberger H (1976) Anal Biochem 70:263–267
Dujon B (1981) In: The molecular biology of the yeast saccharomyces. Life cycle and inheritance. Cold Spring Harbor Laboratory, pp 505–635
Ewing MW, Deters DW (1984) submitted for publication
Fox TD (1979a) J Mol Biol 130:63–82
Fox TD (1979b) Proc Natl Acad Sci USA 76:6534–6538
Fox TD, Leaver CJ (1981) Cell 26:315–323
Freedman JA, Chan SHP (1984) J Bioenerg Biomembr 16:75–100
Hiesel R, Brennicke A (1983) EMBO J 2:2173–2178
Leaver CJ (1982) Ann Rev Plant Physiol 33:373–402
Levings CS (1983) Cell 32:659–661
Macino G, Morelli G (1983) J Biol Chem 258:13230–13235
Maniatis T, Fritsch EF, Sambrook J (1982) In: Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory
Meyer R, Hinds M, Brasch M (1982) J Bacteriol 150:552–562
Poyton RO, Schatz G (1975) J Biol Chem 250:762–766
Sanders JPM, Flavell RA, Borst P, Mol JNM (1973) Biochim Biophys Acta 313:441–457
Sanger F, Nicklen S, Coulson RA (1977) Proc Natl Acad Sci USA 74:5463–5467
Schatz G, Butow R (1983) Cell 32:316–318
Southern EM (1975) J Mol Biol 98:503–512
Steffens GJ, Buse G (1979) Hoppe-Seyler's Z Physiol Chem 360:613–619
Wickerham LJ (1970) In: Lodder J (eded) The yeasts. A taxonomic study. North Holland Publishing Co, pp 227–245, 299–302
Wikstrom M, Krab K, Saraste M (1981) In: Cytochrome oxidase —A synthesis. Academic Press
Williamson DH, Fennell DJ (1975) Methods Cell Biol 12:335–351
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Lawson, J.E., Deters, D.W. Identification and isolation of the cytochrome oxidase subunit II gene in mitochondria of the yeast Hansenula saturnus . Curr Genet 9, 345–350 (1985). https://doi.org/10.1007/BF00421604
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DOI: https://doi.org/10.1007/BF00421604