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Analysis of 6 VNTR loci by ‘multiplex’ PCR and automated fluorescent detection

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Abstract

The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to ‘allelic dropout’ was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

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Authors and Affiliations

  1. Central Research and Support Establishment, Home Office Forensic Science Service, Aldermaston, RG7 4PN, Reading, Berkshire, UK

    Gillian Tully, Kevin M. Sullivan & Peter Gill

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  1. Gillian Tully
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  2. Kevin M. Sullivan
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  3. Peter Gill
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Tully, G., Sullivan, K.M. & Gill, P. Analysis of 6 VNTR loci by ‘multiplex’ PCR and automated fluorescent detection. Hum Genet 92, 554–562 (1993). https://doi.org/10.1007/BF00420938

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  • DOI: https://doi.org/10.1007/BF00420938

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