Summary
Multiforms of megamodulin-dependent protein kinases (M-PK) were partially purified from baker's yeast by excluding endogenous megamodulin with histone, and then by gel filtration with Sephadex G-200. The stimulation of M-PK in the presence of Mg2+, Mn2− or Co2+ was enhanced by yeast megamodulin. In addition, similar augmented activity of M-PK was also noted in the presence of Mg2− by megamodulins prepared from E. coli, bovine brain and wheat germ.
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References
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Kuo, WN. Multiforms of megamodulin-dependent protein kinases from baker's yeast. Mol Cell Biochem 64, 39–44 (1984). https://doi.org/10.1007/BF00420926
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DOI: https://doi.org/10.1007/BF00420926