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Liver and intestinal fatty acid binding proteins in control and TGFβ1 gene targeted deficient mice

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Abstract

The effect of transforming growth factor beta-1 (TGFβ1) expression on fatty acid binding proteins was examined in control and two strains of gene targeted TGFβ1-deficient mice. Homozygous TGFβ1-deficient 129 × CF-1, expressing multifocal inflammatory syndrome, had 25% less liver fatty acid binding protein (L-FABP) when compared to control mice. The decrease in L-FABP expression was not due to multifocal inflammatory syndrome since homozygous TGFβ1-deficient/immunodeficient C3H mice on a SLID background had 36% lower liver L-FABP than controls. This effect was developmentally related and specific to liver, but not the proximal intestine, where L-FABP is also expressed. Finally, the proximal intestine also expresses intestinal-FABP (1-FABP) which decreased 3-fold in the TGFβ1-deficient/immunodeficient C3H mice only. Thus, TGFβ1 appears to regulate the expression of L-FABP and I-FABP in the liver and the proximal intestine, respectively.

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Abbreviations

L-FABP:

liver fatty acid binding protein

I-FABP:

intestinal fatty acid binding protein

TGFβ1:

transforming growth factor beta-1

TNF-α:

tumor necrosis factor-α

MIP-α:

macrophage inflammatory protein-α

PMSF:

phenylmethyl sulfonyl fluoride

PBS:

phosphate buffered saline

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Authors and Affiliations

  1. Department of Veterinary Pathobiology, Texas A & M University, TVMC, 77843-4467, College Station, TX

    Robert N. Fontaine, Ruanna E. Gossett & Ann B. Kier

  2. Department of Veterinary Physiology and Pharmacology, Texas A & M University, TVMC, 77843-4467, College Station, TX

    Friedhelm Schroeder

  3. Division of Comparative Pathology, Department of Pathology, University of Cincinnati Medical Center, 45267-0529, Cincinnati, OH, USA

    Barbara A. O'Toole

  4. Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, 45267-0529, Cincinnati, OH, USA

    Thomas Doetschman

Authors
  1. Robert N. Fontaine
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  2. Ruanna E. Gossett
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  3. Friedhelm Schroeder
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  4. Barbara A. O'Toole
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  5. Thomas Doetschman
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  6. Ann B. Kier
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Fontaine, R.N., Gossett, R.E., Schroeder, F. et al. Liver and intestinal fatty acid binding proteins in control and TGFβ1 gene targeted deficient mice. Molecular and Cellular Biochemistry 159, 149–153 (1996). https://doi.org/10.1007/BF00420917

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  • DOI: https://doi.org/10.1007/BF00420917

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