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Characterization of monoclonal antibodies directed against distinct conserved epitopes of human immunodeficiency virus type 1 core proteins

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Summary

Two mouse hybridoma cell lines secreting antibodies to the Human Immunodeficiency Virus (HIV) p25 major core protein and its precursors p55 and p41, were developed after immunization with the highly cytopathic Zaïrian HIV-1 isolate, NDK. These monoclonal antibodies also react with the gag gene products from HIV-1-BRU prototype and present cross reaction with HIV-2-ROD, and SIV-AGM. They map into topographically distinct areas of p25 and define epitopic regions topographically separated from those recognized by four other anti-p25 mAb suggesting the existence of at least 6 spatially distinct epitopic regions on HIV-1-p25 core protein.

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Abbreviations

HIV:

Human Immunodeficiency Virus

SIV:

Simian Immunodeficiency Virus

HTLVI:

Human T cell Leukaemia Virus

AIDS:

Acquired Immune Deficiency Syndrome

mAb:

Monoclonal Antibody

ELISA:

Enzyme Linked Immunosorbent Assay

PBS:

Phosphate Buffered Saline

References

  1. Barre-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blon C, Vezinet-Brun F, Rouzioux C, Rozenbaum W, Montagnier L: Isolation of a T-lymphotropic retrovirus from patient at risk for acquired immunodeficiency syndrome (AIDS). Science 220: 868–871, 1983

    Google Scholar 

  2. Gallo RC, Salahuddin SZ, Popovic M, Shearer GM, Kaplan M, Haynes BF, Palker TJ, Redfield, Oleske J, Safai B, White G, Foster P, Markham PD: Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science 224: 500–503, 1984

    Google Scholar 

  3. Alizon M, Wain-Hobson S, Montagnier L, Sonigo P: Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolated from African patients. Cell 46: 63–74, 1986

    Google Scholar 

  4. Guyader M, Emerman M, Sonigo P, Clavel F, Alizon M: Genome organization and transactivation of the human immunodeficiency virus type 2. Nature 326: 662–669, 1987

    Google Scholar 

  5. Daniel MD, Letvin NL, King NW, Kannagi M, Sehgal PK, Hunt RD, Kanki PJ, Essex M, Desrosiers RC: Isolation of T-cell tropic HTLV-III Like Retrovirus from Macaques. Science 228: 1201–1204, 1985

    Google Scholar 

  6. Ohta Y, Masuda T, Tsujimoto H, Ishikawa KI, Kodama T, Morikawa S, Nakai M, Honjo S, Hayami M: Isolation of Simian Immunodeficiency Virus from African Green Monkeys and seroepidemiology survey of the virus in various non human primates. Int J Cancer 41: 115–122, 1988

    Google Scholar 

  7. Spire B, Sire J, Zachar V, Rey F, Barre-Sinoussi F, Galibert F, Hampe A, Chermann JC: Nucleotide sequence of the HIV-1 NDK: a highly cytopathic strain. Gene 81: 275–284, 1989

    Google Scholar 

  8. Fisher AG, Ensoli B, Looney D, Rose A, Gallo RC, Saag MS, Shaw GM, Hahn B, Wong-Staal F: Biologically diverse molecular variants within a single HIV-1 isolate. Nature 334:444–447, 1988

    Google Scholar 

  9. Hahn BH, Gonda MA, Shaw GM, Popovic M, Hoxie JA, Gallo RC, Wong-Staal F: Genomic diversity of acquired immune deficiency syndrome virus HTLV-III: different virus exhibit greatest divergence in their envelope gene. Proc Natl Acad Sci (USA) 82: 4813–4817, 1985

    Google Scholar 

  10. Magasiny S, Spire B, Barre-Sinoussi F, Chermann JC: Genomic variability of selected LAV-related AIDS Retroviruses. AIDS Res 2: 19–30, 1986

    Google Scholar 

  11. Di Marzo Veronese F, Sarnagadharan MG, Rahman R, Markham PD, Popovic M, Bodner AJ, Gallo RC: Monoclonal antibodies specific for p24, the major core protein of human T-cell leukemia virus type III. Proc Natl Acad Sci (USA) 82: 5199–5202, 1985

    Google Scholar 

  12. Chassagne J, Verrelle P, Dionet C, Clavel F, Barre-Sinoussi F, Chermann JC, Montagnier L, Gluckman JC, Klatzmann D: A monoclonal antibody against LAV gag precursor: use for viral protein analysis and antigenic expression in infected cells. J Immunol 136: 1442–1445, 1986

    Google Scholar 

  13. Ferns RB, Tedder RS, Weiss RA: Characterization of monoclonal antibody against the human immunodeficiency virus (HIV) gag products and their used in monitoring HIV isolate variation. J Gen Virol 68: 1543–1551, 1987

    Google Scholar 

  14. Minassian AA, Kalyanaraman VS, Gallo RC, Popovic M: Monoclonal antibodies against human immunodeficiency virus (HIV) type 2 core proteins; Cross-reactivity with HIV type 1 and simian immunodeficiency virus. Proc Natl Acad Sci (USA) 85: 6939–6943, 1988

    Google Scholar 

  15. Niedrig M, Rabanus JP, L'Agestehr J, Gelderblom HR, Pauli G: Monoclonal antibodies directed against Human Immunodeficiency Virus (HIV) gag proteins with specificity for conserved epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus. J Gen Virol 69: 2031–2036, 1988

    Google Scholar 

  16. Asano M, Morikawa S, Tatsumi M, Ohta Y, Ishikawa KI, Ushijima H, Hayami M, Kitamura T: Common epitopes on gag proteins of HIV-1 and SIV (AGM) using monoclonal antibodies. (Abst). IV Int Conf on AIDS, 1988

  17. Laurence J, Brun-Vezinet F, Schutzer SE, Rouzioux C, Klatzmann D, Barre-Sinoussi F, Chermann JC, Montagnier L: Lymphadenopathy associated viral antibody in AIDS. Immune correlations and definition of a carrier state. N England J Med 311: 1269–1273, 1984

    Google Scholar 

  18. Sarngadharan MG, Di Marzo Veronese F, Lee S, Gallo RC: Immunological properties of HTLV-111 antigens recognized by sera of patients of AIDS and AIDS-related complex and of asymptomatic carriers of HTLV-III infection. Cancer Res 45: 4574S–4577S, 1985

    Google Scholar 

  19. Jackson GG, Rubenis M, Knigge M, Perkins IT, Paul DA, Despotes JC, Spencer P: Passive immunoneutralizing of Human Immunodeficiency Virus in patients with advanced AIDS. The Lancet 11: 647–651, 1988

    Google Scholar 

  20. Pierres M, Kourilsky F, Rebouah MP, Dosseto M, Caillol D: Distinct epitopes on Ik gene products identified by monoclonal antibodies. Eur J Immunol 10: 950–957, 1980

    Google Scholar 

  21. Ellrodt A, Le Bras Ph, Brun-Vezinet F, Segond P, Montagnier L, Barre-Sinoussi F, Nugeyre MT, Rey F, Rouzioux C, Caquet R, JC Chermann: Isolation of a human T lymphotropic retrovirus (LAV) from Zaïrian married couple, one with AIDS, one with prodomes. Lancet i: 1383–1385, 1984

    Google Scholar 

  22. Kearney JF, Radbruck A, Liesegang B, Rajewsky K: A new mouse myelorna cell line that has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cell line. J Immunol 123: 1548–1550, 1979

    Google Scholar 

  23. Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC: Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266: 550–552, 1977

    Google Scholar 

  24. Frazekas de St Groth S, Scheidegger D: Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35: 1–22, 1980

    Google Scholar 

  25. Devaux CA, Nadler PI, Miller GG, Sachs DH: Genetic control of immune response to staphylococcal nuclease. XII: Analysis of nuclease antigenic determinants using antinuclease monoclonal antibodies. Mol Cell Biochem 68: 31–40, 1985

    Google Scholar 

  26. Chiba J, Nishimura M, Nagata S, Sata T, Morikawa S, Kurata T: A monoclonal antibody useful for detection of HIV in acetone fixed cells. (Abst). IVthe Int Conf on AIDS, 1988

  27. Popovic M, Lange-Wantzin G, Sarin PS, Mann D, Gallo RC: Transformation of human umbilical cord blood T cells by human T cell leukemia/lymphoma virus. Proc Natl Acad Sci USA 80: 5402–5406, 1980

    Google Scholar 

  28. Laemli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–683, 1970

    Google Scholar 

  29. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci (USA) 76: 4350–4354, 1979

    Google Scholar 

  30. Rey MA, Spire B, Dormont D, Barre-Sinoussi F, Montagnier L, JC Chermann: Characterization of the RNA dependant DNA polymerase of a new human T lymphotropic retrovirus (LAV). Biochem Biophys Res Comm 121: 126–133, 1984

    Google Scholar 

  31. Rey F, Barre-Sinoussi F, Chermann JC: Biosynthesis of LAV gag gene products: presence of an intracellular gagpol precursor polyprotein. Ann Inst Pasteur Virol 138: 161–168, 1987

    Google Scholar 

  32. Kramer RA, Schaber MD, Slalka AM, Ganguly K, Wong-Staal F, Reddy EP: HTLV-III gag protein is processed in yeast cells by the virus pol-protease. Science 231: 1580–1585, 1986

    Google Scholar 

  33. Evans LA, Homsy JM, Morrow WJW, Gaston I, Sooy CD, Levy J: Human monoclonal antibody directed against gag gene products of the Human Immunodeficiency Virus. J Immunol 140: 941–943, 1988

    Google Scholar 

  34. Parekh B, Leverah J, Bredt B: Immunodeficiency virus (HIV-1) p24 and its antigenic sites using monoclonal antibodies. (Abst). IV Int Conf on AIDS, 1988

  35. Mehta S, Kennedy DW, Aneja R, Devare SC, Hunt JC: Monoclonal antibodies to HIV-1 p24 recognize antigenically cross reacting epitopes of HIV-2 p24. (Abst). 4th Int Conf on AIDS, 1988

  36. Tersmette M, Winkel IN, Groenink M, Gruters RA, Spence RP, Saman E, Van der Groen G, Miedema F, Huisman JG: Detection and subtyping of HIV-1 isolates with a panel of characterized monoclonal antibodies to HIV p24 gag. Virology 171: 149–155, 1989

    Google Scholar 

  37. Jackson GG, Paul DA, Falk LA: Human Immunodeficiency Virus (HIV) antigenemia (p24) in the acquired immunodeficiency syndrome (AIDS) and the effect of treat ment with zidovudine (AZT). Ann Intern Med 108: 175–180, 1988

    Google Scholar 

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Authors and Affiliations

  1. Unité INSERM de Recherches sur les Rétrovirus et Maladies Associeés (U.322), Campus Universitaire de Luminy, B. P. 33, 13273, Marseille Cédex 9, France

    Masashi Tatsumi, François Kourilsky & Jean-Claude Chermann

  2. CRBM du CNRS, Centre de Tri des Molecules anti-HIV, Boulevard Henri IV, 34060, Montpellier Cedex, France

    Christian Devaux

Authors
  1. Masashi Tatsumi
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  2. Christian Devaux
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  3. François Kourilsky
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  4. Jean-Claude Chermann
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Tatsumi, M., Devaux, C., Kourilsky, F. et al. Characterization of monoclonal antibodies directed against distinct conserved epitopes of human immunodeficiency virus type 1 core proteins. Mol Cell Biochem 96, 127–136 (1990). https://doi.org/10.1007/BF00420904

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  • DOI: https://doi.org/10.1007/BF00420904

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