Summary
Mutants resistant to 200 µg/ml of the alkaloid lycorine (LYC R) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYC R/lyc s) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the resistant phenotype. In the wild type total protein synthesis is only slightly inhibited, whereas DNA and RNA synthesis is lowered to about 30% of the control. In the lycorine resistant mutants all macromolecular syntheses are unaffected by the drug.
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References
Arrigoni O, Arrigoni-Liso R, Calabrese G (1975) Nature 256:513–514
Del Giudice L, Puglisi PP (1974) Biochem Biophys Res Commun 59:865–871
Del Giudice L, Massardo DR, Manna F, Evidente A, Randazzo G, Wolf K (1984) Current Genetics 8:493–498 (Erratum: Current Genetics 9:183 (1985))
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Dedicated to Prof. Dr. Fritz Kaudewitz on the occasion of his 65th birthday
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Del Giudice, L., Massardo, D.R., Manna, F. et al. Isolation and genetical and biochemical characterization of mutants resistant to the alkaloid lycorine. Curr Genet 11, 247–249 (1986). https://doi.org/10.1007/BF00420614
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DOI: https://doi.org/10.1007/BF00420614