Summary
Drosophila imaginal disc cell lines show a characteristic pattern of aggregation in culture, which appears to be due to cell-cell rather than cell-substrate interactions. We have examined the distribution of PS integrins in wing and leg cell lines, and find that these integrin homologues are expressed preferentially in aggregates. Cell sheets, small cell clumps and chains of cells express antigen at points of cell-cell contact only.
This is a preview of subscription content, log in via an institution to check access.
References
Bogaert T, Brown N, Wilcox M (1987) The Drosophila PS2 antigen is an invertebrate integrin that, like the fibronectin receptor, becomes localised to muscle attachments. Cell 51:929–940
Brower DL, Piovant M, Reger LA (1985) Developmental analysis of Drosophila position-specific antigens. Dev Biol 108:120–130
Brower DL, Wilcox M, Piovant M, Smith RJ, Reger LA (1984) Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs. Proc Natl Acad Sci USA 81:7485–7489
Carter WG, Wayner EA, Bouchard TS, Kaur P (1990) The role of integrins alpha-2 beta-1 and alpha-3 beta-1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol 110:1387–1404
Cullen CF, Milner MJ (1991) Parameters of growth in primary cultures and cell lines established from Drosophila imaginal discs. Tissue and Cell 23:29–39
Currie DA, Milner MJ, Evans CW (1988) The growth and differentiation in vitro of leg and wing imaginal disc cells from Drosophila melanogaster. Development 102:805–814
Hynes RD (1987) Integrins: a family of cell surface receptors. Cell 48:549–554
Kaufman R, Frosch D, Westphal C, Weber L, Klein CE (1989) Integrin VLA-3: ultrastructural localisation at cell-cell contact sites of human cell cultures. J Cell Biol 109:1807–1815
Larjava H, Peltonen J, Akiyama SK, Yamada SS, Gralnick HR, Uitto J, Yamada KM (1990) Novel function for beta-1 integrins in keratinocyte cell-cell interactions. J Cell Biol 110:803–815
Leptin M, Aebersold R, Wilcox M (1987) Drosophila positionspecific antigens resemble the vertebrate fibronectin-receptor family. EMBO J 6:1037–1043
Leptin M, Bogaert T, Lehmann R, Wilcox M (1989) The function of PS integrins during Drosophila embryogenesis. Cell 56:401–408
O'Brochta DA, Bryant PJ (1985) A zone of non-proliferating cells at a lineage restriction boundary in Drosophila. Nature 313:138–141
Peel DJ (1991) Cell interactions and the response to ecdysteroids of Drosophila imaginal disc cell lines. PhD Thesis, University of St. Andrews
Peel DJ, Johnson SA, Milner MJ (1990) The ultrastructure of imaginal disc cells in primary cultures and during cell aggregation in continuous cell lines. Tissue and Cell 22:749–758
Peel DJ, Milner MJ (1990) The diversity of cell morphology in cloned cell lines derived from Drosophila imaginal discs. Roux's Arch Dev Biol 198:479–482
Springer TA (1990) Adhesion receptors of the immune system. Nature 346:425–434
Takeichi M (1988) The cadherins: cell-cell adhesion molecules controlling animal morphogenesis. Development 102:639–655
Volk T, Fessler LI, Fessler JH (1990) A role for integrin in the formation of sarcomeric cytoarchitecture. Cell 63:525–536
Wilcox M, Brower DL, Smith RJ (1981) A position-specific cell surface antigen in the Drosophila wing imaginal disc. Cell 25:159–164
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peel, D.J., Milner, M.J. The expression of PS integrins in Drosophila melanogaster imaginal disc cell lines. Roux's Arch Dev Biol 201, 120–123 (1992). https://doi.org/10.1007/BF00420423
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00420423