Summary
We have characterized a cDNA clone designated XSYO, complementary to a transcript that is highly expressed during Xenopus oogenesis. XSYO is expressed as a maternal mRNA in oocytes and early embryos at a level up to 3 × 108 copies per mature oocyte. This level is 100-fold higher than the concentration of an average maternal RNA in the oocyte and close to the value found for the stockpiled maternal histone mRNA. This level remains constant thoughout the first rapid cleavage stages until the midblastula transition (MBT). After this stage, the XSYO RNA is degraded within 2 h and, after the blastula stage, a very weak expression was detected. This gene is not expressed in Xenopus proliferative somatic cultured cells, suggesting that it is not a simple housekeeping gene. The presence of a potential metal-binding domain in the XSYO sequence suggests that this gene might code for a protein involved in nucleic acid binding or gene regulation specific to oogenesis or early development.
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Andéol Y, Gusse M, Méchali M (1990) Characterization and expression of a Xenopus RAS during oogenesis and development. Dev Biol 139:24–34
Anderson DM, Richter JD, Chamberlin ME, Price DH, Britten RJ, Smith LD, Davidson EH (1982) Sequence organization of the poly(A) RNA synthesized and accumulation in lampbrush chromosome stage Xenopus laevis oocytes J Mol Biol 155:281–309
Berg JM (1986) Potential metal-binding domains in nucleic acid binding proteins. Science 232:485–487
Bisbee CA, Baker MA, Wilson AC, Hadji-Azimi I, Fischberg M (1977) Albumin phylogeny for clawed frogs (Xenopus). Science 185:785–787
Davidson EH (1986) Gene activity in early development. Academic Press
Dawid IB (1965) Deoxyribonucleic acid in amphibian eggs. J Mol Biol 12:581–599
Dumont JN (1972) Oogenesis in Xenopus laevis (Daudin): I. Stages of oocyte development in laboratory maintained animals. J Morphol 136:153–164
Dworkin MB, Dawid I (1980) Use of a cloned library for the study of abundant polyA+ RNA during Xenopus laevis development. Dev Biol 76:449–464
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction encodnuclease. Anal Biochem 132:6–13
Friend SH, Horowitz JM, Gerber MR, Wang XF, Bogenmann E, Li P, Weinberg RA (1987) Deletions of a DNA sequence in retinoblastomas and mesenchymal tumors: organization of the sequence and its encoded protein. Proc Natl Acad Sci USA 84:9059–9063
Gurdon JB (1967) African clawed frogs. In: Wilt FH, Wessels NK (eds) Crowell, New York, pp 75–84
Gurdon JB, Wickens MP (1983) The use of Xenopus oocytes for the expression of cloned genes. Methods Enzymol 101:370–386
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted break points for DNA sequencing. Gene 28:351–359
Jacob L, Opper M, Metzroth B, Phannavong B, Mechler BM (1987) Structure of the 1(2)gl gene of Drosophila and delimitation of its tumor suppressor domain. Cell 50:215–225
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Miller J, McLachlan AD, Klug A (1985) Mutations within the 23S rRNA coding sequence of E. coli with block ribosome assembly. EMBO J 4:1602–1609
Moreau J, Matyask-Smirniaguina L, Scherrer K (1981) Systematic punctuation of eukaryotic DNA by A+T-rich sequences. Proc Natl Acad Sci USA 78:1341–1345
Newport JW, Kirschner M (1982) A major developmental transition in early Xenopus embryos: I. Characterization and timing of cellular changes at the midblastula stage cell. Cell 30:675–686
Nieuwkoop PD, Faber J (1956) Normal table of Xenopus laevis (Daudin). North-Holland Publishing, Amsterdam
Rebagliati MR, Weeks DL, Harvey RP, Melton DA (1985) Identification and cloning of localized maternal RNAs from Xenopus eggs. Cell 42:769–777
Rosbash M, Ford PJ (1974) Polyadenylic acid-containing RNA in Xenopus laevis oocytes. J Mol Biol 85:87–101
Sagata M, Oskarsson M, Copeland T, Brumbaugh J, VandeWoude GF (1988) Function of c-mos proto-oncogene product in meitoc maturation in Xenopus oocytes. Nature 335:519–525
Sanger F, Nicklens S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Smith D, Richter JD, Taylor MA (1984) Regulation of translation during oogenesis. In: Davidson EH, Firstel RA (eds) Molecular approaches to developmental biology. A R Liss, Inc. New York, pp 129–143
Taylor MV, Gusse M, Evan GI, Dathan N, Méchali M (1986) Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division. EMBO J 5:3563–3570
Vriz S, Taylor M, Méchali M (1989) Differential expression of two Xenopus c-myc proto-oncogenes during development. EMBO J 8:4091–4097
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Vriz, S., Andéol, Y. & Méchali, M. Isolation and developmental expression of an oogenesis-specific Xenopus cDNA clone. Roux's Arch Dev Biol 201, 113–119 (1992). https://doi.org/10.1007/BF00420422
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DOI: https://doi.org/10.1007/BF00420422