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Coding capacity of poly(A)+ mRNA isolated from mengovirus-infected Ehrlich ascites tumor cells

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Abstract

Total poly(A)+ mRNA was isolated from mengovirus-infected Ehrlich ascites tumor cells at various times postinfection and quantitated in a cell-free system derived from uninfected ascites cells. Basic proteins were separated from acidic proteins by carboxymethyl cellulose chromatography. At the end of the infectious cycle, 8 h postinfection, the cellular contents of most mRNAs coding for basic ribosomal proteins are still between 70 and 90 per cent of those measured at the beginning of infection or in uninfected cells. On the basis of this result, the rapid shutoff of host protein synthesis after mengovirus infection of Ehrlich ascites tumor cells cannot be the consequence of the inactivation of host template RNA.

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Author notes

  1. P. B. Hackett

    Present address: Department of Microbiology, University of California, 94143, San Francisco, Calif., USA

  2. E. Egberts

    Present address: Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Hollandseweg 13, Wageningen, The Netherlands

Authors and Affiliations

  1. Max-Planck-Institut für Zellbiologie, 2940, Wilhelmshaven, Germany

    P. B. Hackett, E. Egberts, I. Buchwald & P. Traub

Authors
  1. P. B. Hackett
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  2. E. Egberts
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  3. I. Buchwald
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  4. P. Traub
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Hackett, P.B., Egberts, E., Buchwald, I. et al. Coding capacity of poly(A)+ mRNA isolated from mengovirus-infected Ehrlich ascites tumor cells. Mol Biol Rep 3, 387–396 (1977). https://doi.org/10.1007/BF00420398

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  • DOI: https://doi.org/10.1007/BF00420398

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