Abstract
Electrophoretic mobility of histones from cell cultures of Drosophila melanogaster and of the mosquito Aedes aegypti was determined in polyacrylamide gels in the presence of different concentrations of urea. Great similarity in the electrophoretic behavior of H3, H2A, H2B and H4 histones from the two insect species was found. Histone H1 of Aedes under all conditions tested had a markedly higher electrophoretic mobility than H1 of Drosophila, but differed only slightly from H1 histones of mouse and of hamster.
As can be deduced from the mobility of Aedes H1 in the presence of sodium dodecyl sulphate its molecular weight is smaller than that of Drosophila H1 and is very close to the molecular weight of the main component of mouse H1 histone. Heterogeneity of the H1 histone from Drosophila is demonstrated. This heterogeneity is due to phosphorylation of a part of H1 molecules, since it disappears after the treatment of H1 preparations by alkaline phosphatase. Phosphorylated components were not found in the H1 of Aedes.
Thus two representatives of Diptera, Aedes and Drosophila possessing polytene chromosomes at the larval stage of development have H1 histones with markedly different primary structures. This pact demonstrates that the polytenization of chromosomes may occur in species with markedly different H1 histones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
ElginS. C. R. and WeintraubH., Ann. Rev. Biochem 44, 725 (1975).
PanyimS., BilekD., and ChalkleyR., J. Biol. Chem. 246, 4206 (1971).
PanyimS. and ChalkleyR., J. Biol. Chem. 246, 7557 (1971).
KinkadeJ. M.Jr. and ColeR. D., J. Biol. Chem. 241, 5790 (1966).
KinkadeJ. M.Jr. and ColeR. D., J. Biol. Chem. 241, 5798 (1966).
KinkadeJ. M., J. Biol. Chem. 244, 3375 (1969).
BustinM. and ColeR. D., J. Biol. Chem. 243, 4500 (1968).
BustinM. and ColeR. D., J. Biol. Chem. 244, 5286 (1969).
LanganT. A., RallS. C., and ColeR. D., J. Biol. Chem. 246, 1942 (1971).
BustinM. and StollarB. D., J. Biol. Chem. 248, 3506 (1973).
BustinM. and ColeR. D., J. Biol. Chem. 244, 5291 (1969).
RallS. C. and ColeR. D., J. Biol. Chem. 246, 7175 (1971).
SherodD., JohnsonG. and ChalkleyR., J. Biol. Chem. 249, 3923 (1974).
JonesG. M. T., RallS. C. and ColeR. D., J. Biol. Chem. 249, 2548 (1974).
StricklandW. N., SchallerH., StricklandM. and VonHoltC., FEBS Letters 66, 322 (1976).
CohenL. H. and GotchelB. V., J. Biol. Chem. 246, 1841 (1971).
OliverD. and ChalkleyR., Exp. Cell Res. 73, 295 (1972).
OliverD. and ChalkleyR., Exp. Cell Res. 73, 303 (1972).
FrancoL., MonteroF., NavletJ. M., PereraJ. and RojoM. C., Eur. J. Biochem. 48, 53 (1974).
AlfagemeC. R., ZweidlerA., MahowaldA., and CohenL. H., J. Biol. Chem. 249, 3729 (1974).
HolmgrenP., RasmusonB., JohanssonT., and SundquistG., Chromosoma 54, 99 (1976).
SubiranaJ. A., PalauJ., CozcolluelaC., and Ruiz-CarrilloA., Nature 228, 992 (1970).
SmithE. L., DeLangeR. J., and BonnerJ., Physiol. Rev. 50, 159 (1970).
PolukarovaL. G., KakpakovV. T., and GvozdevV. A., Genetica, USSR XI, No. 5, 46 (1975).
PanyimS. and ChalkleyR., Arch. Biochem. Biophys. 130, 337 (1969).
PanyimS. and ChalkleyR., Biochemistry 8, 3972 (1969).
SchaffnerW. and WeissmannC., Anal. Biochem. 56, 502 (1973).
McMaster-KayeR. and KayeJ. S., Arch. Biochem. Biophys. 156, 426 (1973).
SherodD., JohnsonG., and ChalkleyR., Biochemistry 9, 4611 (1970).
CohenL. H., NewrockK. M., and ZweidlerA., Science 190, 994 (1975).
Author information
Authors and Affiliations
Additional information
at Moscow
at Nijmegen
Rights and permissions
About this article
Cite this article
Bogdanova, E.S. Comparative electrophoretic properties of histones from cells of the mosquito Aedes aegypti and of the fruitfly Drosophila melanogaster. Mol Biol Rep 3, 361–369 (1977). https://doi.org/10.1007/BF00420395
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00420395