Summary
A DNA fragment which carries the leul gene encoding beta-isopropylmalate dehydrogenase in Schizosaccharomyces pombe has been isolated by complementation of an E. coli leuB mutation. This 1.5 kb DNA fragment complements not only the S. pombe leul mutation, but also the S. cerevisiae leu2 mutation. The nucleotide sequence of the essential part of the leul gene and its flanking regions was determined. This sequence contains an open reading frame of 371 codons, from which a protein having a Mr = 39,732 can be predicted. The deduced amino acid sequence and its codon usage were compared with those of the S. cerevisiae LEU2 protein. The cloned DNA will be a useful marker when transforming S pombe.
Similar content being viewed by others
References
Andreadis A, Hsu Y-P, Hermodson M, Kohlhaw G, Schimmel P (1984) J Biol Chem 259:8059–8062
Beach D, Nurse P (1981) Nature 290:140–142
Beggs JB (1978) Nature 275:104–109
Bennetzen JL, Hall BD (1982) J Biol Chem 257:3026–3031
Botstein DS, Falco C, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Gene 8:17–23
Davidow LS, Kaczmarek FS, DeZeeuw JR, Colon SW, Lauth MR, Pereira DA, Franke AE (1987) Curr Genet 11:377–383
Ikemura T (1982) J Mol Biol 158:573–597
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Kagawa Y, Nojima H, Nukiwa N, Ishizuka M, Nakajima T, Yasuhara T, Tanaka T, Oshima T (1984) J Biol Chem 259:2956–2960
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Martinez-Arias AE, Casadaban MJ (1983) Mol Cell Biol 3:580–586
Messing J (1983) Methods Enzymol 101:20–78
Proudfoot NJ, Brownlee GG (1976) Nature 263:211–214
Ratzkin B, Carbon J (1977) Proc Natl Acad Sci USA 74:487–491
Sakaguchi J, Yamamoto M (1982) Proc Natl Acad Sci USA 79:7819–7823
Sanger F (1981) Science 214:1205–1210
Sekiguchi T, Ortega-Cesena J, Nosoh Y, Ohashi S, Tsuda K, Kanaya S (1986) BBA 867:36–44
Sherman F, Fink GR, Hicks JB (1981) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Takagi M, Kobayashi N, Sugimoto M, Fujii T, Watari J, Yano K (1987) Curr Genet 11:451–457
Uemura T, Yanagida M (1984) EMBO J 3:1737–1744
Uemura T, Yanagida M (1986) EMBO J 5:1003–1010
Yamamoto M, Asakura Y, Yanagida M (1981) Mol Gen Genet 182:426–429
Zaret KS, Sherman F (1982) Cell 28:563–573
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kikuchi, Y., Kitazawa, Y., Shimatake, H. et al. The primary structure of the leul + gene of Schizosaccharomyces pombe . Curr Genet 14, 375–379 (1988). https://doi.org/10.1007/BF00419995
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00419995