Skip to main content
Log in

The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

We have isolated and characterized the F0-ATPase subunit 6 gene (atp6) from tobacco mitochondria. The tobacco sequence exists as a single copy, is transcribed and contains an open reading frame (ORF) capable of encoding a peptide of 395 amino acids. The first 130 amino acids of the tobacco putative polypeptide show limited homology with the N terminus predicted for the maize ATPase subunit 6. Although poorly conserved at the sequence level, the tobacco and maize amino termini are hydrophilic and have a high percentage of charged amino acids. This portion of the predicted peptide may represent a presequence that is common to the ATPase subunit 6 of plants. Significant homology between tobacco and maize begins with amino acid 131, in a region that is highly conserved among fungal ATPase 6 subunits. In the remainder of the predicted protein, tobacco and maize share approximately 81% homology. A 41 by sequence and a 175 by conserved region found upstream from the tobacco atp6 coding region are homologous with sequence elements found in the 5′ flanking regions of other plant mitochondrial genes and may be important for regulation and expression of the atp6 gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bland MM, Levings CS III, Matzinger DF (1986) Mol Gen Genet 204:8–16

    Google Scholar 

  • Bland MM, Matzinger DF, Levings CS III (1985) Theor Appl Genet 69:535–541

    Google Scholar 

  • Braun CJ, Levings CS III (1985) Plant Physiol 79:571–577

    Google Scholar 

  • Brennicke A, Moller S, Blanz PA (1985) Mol Gen Genet 198: 404–410

    Google Scholar 

  • Chao S, Sederoff R, Levings CS III (1984) Nucleic Acids Res 12:6629–6644

    Google Scholar 

  • Clary DO, Wolstenholme DR (1983) Nucleic Acids Res 11: 4211–4227

    Google Scholar 

  • Dale RMK, Mendu N, Ginsburg H, Krindl JC (1984) Plasmid 11:141–150

    Google Scholar 

  • Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113

    Google Scholar 

  • Dewey RE, Levings CS III, Timothy DH (1985a) Plant Physiol 79:914–919

    Google Scholar 

  • Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449

    Google Scholar 

  • Dewey RE, Schuster AM, Levings CS III, Timothy DH (1985b) Proc Natl Acad Sci USA 82:1015–1019

    Google Scholar 

  • Eckenrode VK, Levings CS III (1986) In Vitro Cell Devel Biol 22:169–176

    Google Scholar 

  • Fillingame RH (1980) Ann Rev Biochem 49:1079–1113

    Google Scholar 

  • Goldberg DA (1980) Proc Natl Acad Sci USA 77:5794–5798

    Google Scholar 

  • Grabau EA (1986) Plant Mol Biol 7:377–384

    Google Scholar 

  • Grisi E, Brown TA, Waring RB, Scazzocchio C, Davies RW (1982) Nucleic Acids Res 10:3531–3539

    Google Scholar 

  • Hack E, Leaver CJ (1983) EMBO J 2:1783–1789

    Google Scholar 

  • Hiesel R, Brennicke A (1983) EMBO J 2:2173–2178

    Google Scholar 

  • Hiesel R, Schobel W, Schuster W, Brennicke A (1987) EMBO J 6:29–34

    Google Scholar 

  • Houchins JP, Ginsburg H, Rohrbaugh M, Dale RMK, Schardl CL, Hodge TP, Lonsdale DM (1986) EMBO J 5:2781–2788

    Google Scholar 

  • John UP, Willson TA, Linnane AW, Nagley P (1986) Nucleic Acids Res 14:7437–7451

    Google Scholar 

  • Macino G, Tzagoloff A (1980) Cell 20:507–517

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) In: Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Moon E, Kao T-H, Wu R (1985) Nucleic Acids Res 13:3195–3212

    Google Scholar 

  • Morelli G, Macino G (1984) J Mol Biol 178:491–507

    Google Scholar 

  • Neupert W, Schatz G (1981) Trends Biochem Sci 6:1–4

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schuster W, Brennicke A (1985) Curr Genet 9:157–163

    Google Scholar 

  • Schuster W, Brennicke A (1986) Mol Gen Genet 204:29–35

    Google Scholar 

  • Sebald W, Hoppe J, Wachter E (1979) In: Quagliarello E (ed) Function and molecular aspects of biomembrane transport. Elsevier/North Holland Biomedical, Amsterdam, pp 63–74

    Google Scholar 

  • Shine J, Dalgarno L (1974) Proc Natl Acad Sci USA 71:1342–1346

    Google Scholar 

  • Spencer DF, Schnare MN, Gray MW (1984) Proc Natl Acad Sci USA 81:493–497

    Google Scholar 

  • Tzagoloff A (1982) In: Mitochondria. Plenum Press, NY

    Google Scholar 

  • Young EG, Hanson MR, Dierks PM (1986) Nucleic Acids Res 14:7995–8006

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bland, M.M., Levings, C.S. & Matzinger, D.F. The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence. Curr Genet 12, 475–481 (1987). https://doi.org/10.1007/BF00419555

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00419555

Key words

Navigation