Abstract
Major outer membrane proteins of Eikenella corrodens, an organism frequently isolated from patients with periodontal disease, were tested for some biological activities. Mouse peritoneal macrophages, exposed at low concentrations of the above-mentioned proteins (between 0.05 and 5 μg/ml), showed evident and marked morphological modifications consisting of increases in the size and vacuolation of the cells. Higher concentrations showed a toxic effect. Low concentrations resulted in a selective release of lysosomial enzymes without any significant release of lactatedehydrogenase, and cytoplasmic marker; while concentrations of 25–50 μg/ml, which were toxic in trypan-blue exclusion test, increased LDH release.
Eikenella corrodens major proteins increased the platelet aggregation of ADP and thrombin.
The residual complement activity of serum samples incubated with various amounts of proteins at 37°C for 30 minutes appeared strongly reduced with respect to controls, thus showing a consumption of the complement components.
These results suggested that Eikenella corrodens major proteins may play a role in the development of periodontal lesions.
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References
Allison A.C., Schorlemmer H.U. and Bitter-Suermann D. (1976): Activation of complement by the alternative pathway as a factor in the pathogenesis of periodontal disease. -Lancet ii; 1001–1010.
Attstrom R. (1971): Studies on neutrophil polymorphonuclear leukocytes at the dento-gingival junction in gingival health and disease. -J. Periodontal. Res. Suppl. 8, 1–15.
Bang J., Cimasoni G. and Held A.J. (1970): Betaglucuronidase correlated with inflammation in the exudate from human gingiva. -Arch. Oral. Biol. 15, 445–451.
Baumgartener H.R. and Born G.V.R. (1968): Effect of 5-hydroxytryptamine on platelet aggregation. -Nature 218, 135–141.
Bessler W. and Ottenbreitt E. (1977): Studies on the mitogenic principle of lipoprotein from the outer membrane of E. coli. -Biochem. Biophys. Res. Comm. 76, 239–246.
Born G.V.R. (1962): Aggregation of blood platelets by adenosine diphosphate and its reversal. -Nature 194, 927–931.
Bradley S.G. (1979): Cellular and molecular mechanisms of action of bacterial endotoxins. -Annu. Rev. Microbiol. 33, 67–94.
Clark R.A., Page R.C. and Wilde G. (1977): Defective neutrophil chemotaxis in juvenile periodontitis. -Infect. Immun. 18, 694–700.
Cowley G.C. (1972): Enzyme activity in gingival immunocytes. -J. Dent. Res. 51, suppl. 2, 284–292.
Darwish S., Hyppa T. and Socransky S.S. (1978): Studies of the predominant cultivable microbiota of early periodonlitis. -J. Periodon. Res. 13, 1–16.
Fine D.H., Tabak L., Salkind A. and Oshrain H. (1978): Studies in plaque pathogenecity II. A technique for the specific detection of endotoxin in plaque samples using the Limulus lysate assay. -J. Periodon. Res. 13, 127–133.
Galdiero F., Tufano M.A., Rossi F., Filippelli W., Del Gaudio F., Berrino L. and Marino E.( ): Effect of Salmonella typhimurium porins on the cardiovascular and renal apparatus. - Res. Comm. Path. Pharmacol., in press.
Galdiero F., Tufano M.A., Sommese L., Folgore A. and Tedesco F. (1984): Activation of the complement system by porins extracted from Salmonella typhimurium. -Infect. Immun. 46, 559–563.
Goldman C.R., While D. and Leive L. (1981): Identification of outer membrane proteins, including known lymphocyte mitogens, as the endotoxin protein of Escherichia coli 0111. -J. Immunol. 127, 1290–1297.
Goodman G.W. and. Sultzer B. (1979): Characterization of the chemical and physical properties of a novel B-lymphocyte activator, endotoxin protein. -Infect. Immun. 24, 685–689.
Hancock R.E.W. and Nikaido H. (1978): Outer membranes of gram-negative bacteria XIX Isolation from Pseudomonas aeruginosa PA01 and use in reconstitution and definition of the permeability barrier. -J. Bacteriol. 136, 381–390.
Hausman E. (1974): Potential pathways for bone resorption in human periodontal disease. -J. Periodontol. 45, 338–343.
Hausman E., Weinfeld N. and Miller W.A. (1972): Effects of lipopolysaccharide on bone resorption in tissue culture. -Cale. Tiss. Res. 9, 272–282.
Johnson D.A., Behling U.H., Listgarten M. and Nowotny A. (1978): Role of bacterial products in periodontitis: humoral immune response to Eikenella corroderas. -Infect. Immun. 22, 382–386.
Johnson D.A., Lo Chen C., Dombrowski J.C. and Nowotony A. (1976): Role of bacterial products in periodontitis. I Endotoxin content and immunogenicity of human plaque. -J. Periodont Ress. 11, 549–559.
Kiley P. and Holt S.C. (1980): Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. -Infect Immun. 30, 862–873.
Lachmann P.J. and Hobart M.J. (1978): Complerrlent technology. Chapter 5a in D.M. Weir (ed), Handbook of experimental immunology, 3rd ed. Blackwell, Oxford.
Laemmli U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. -Nature (London) 277, 680–685.
Listgarten M.A. (1976): Structure of the microbial flora associated with periodontal disease and health in man. A light and microscopic study. -J. Periodontol. 47, 1–18.
Lowry O.M., Rosebrough N.J., Farr A.L. and Randall R.J. (1951): Proteins measurement with the folio phenol reagent. -J. Biol. Chem. 193, 265–275.
Maliszewski C.R., Shuster C.W. and Badger S.J. (1983):A type specific antigen of Eikenella corrodens is the major outer membrane protein. -Infect. Immun. 42, 208–213.
Marmno E., Caputi A.P., Vacca C. and Cazzola M. (1974): Interferenza tra alcuni antiflogistici e aggregazione delle piastrine. -Riv. Farmacol. Ter. 5, 279–281.
Mergenhagen S.E., Tempel T.R. and Snyderman R. (1970): Immunologic reactions and periodontal inflammation. -J. Den. Res. Suppl. 49, 256–261.
Mills D.C.B. and Roberts G.C.K. (1967): Membrane active drugs and the aggregation of human blood platelets. -Nature 213, 35–42.
Morrison D.C. and Ulevitch R.J. (1978): The effects of bacterial endotoxins on host mediation systems. -Am. J. Path. 93, 527–617.
Newman M.G. and Socransky S.S. (1977): Predominant cultivable microbiota in periodontosis. -J. Periodont. Res. 12, 120–128.
Page R.C., Davies P. and Allison A.C. (1973): Effects of dental plaque on the production and release of lysosomial hydrolases by macrophages in culture. -Arch. Oral. Biol. 18, 1481–1495.
Page R.C. and Schroeder H.E. (1976): Pathogenesis of inflammatory periodontal disease. A summary of current work. -Lab. Investig. 33, 235–249.
Progulske A., Mishell R., Trumrnel C. and Holt S.C. (1984): Biological activities of Eikenella corroderas outer membrane and lipopolysaccharide. -Infect. Immun. 43, 178–182.
Schroeder H.E. (1973): Transmigration and infiltration of leukocytes in human junctional epithelium. -Odont. Acta 17, 6–18.
Socransky S.S. (1977): Microbiology of periodontal disease present status and future considerations. -J. Periodontol. 48, 497–504.
Taichman N.S. (1970): Mediation of inflammation by the polymorphonuclear leokocyte as a sequel of immune reactions. -J. Periodontol 11, 37–43.
Taichman N.S., Mc Arthur W.P. (1975): Current concepts in periodontal disease. -Annu. Rep. Med. Chem. 10, 228–239.
Thye Yin E., Galanos C., Kinsky S., Brodshaws R.A., Wessler S., Luderity O. and Sarmiento M.F. (1972): Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharide and Lipid A from Gram-negative bacteria. -Biochim. Biophys. Acta 26, 284–289.
Tufano M.A., Berlingieri M.T., Sommese L. and Galdiero F. (1984): Immune response in mice and affect on cells by outer membrane porins from Salmonella typhimurium. -Microbiol. 7, 353–366.
Tufano M.A., Marfella A., Galdiero F., Vairo G. and Marmo E. (1983): Effetti delle porine di Salmonella typhimurium sulla aggregazione delle piastrine: ricerche in vivo ed in vitro. - XX Congresso Nazionale Microbiologia; Brescia-Gardone Riviera, Maggio.
Tufano M.A., Rossano F. and Galdiero F. (1982): Effects of porins from Salmonell typhimurium on the mouse: Immunomodulation and some functional systemic modifications. FEMS Symposium on bacterial and viral inhibition and modulation of host defences. - Pisa, September.
Weinberg J.B., Ribi E. and Wheat R.W. (1983): Enhancement of macrophage-mediated tumor killing by bacterial outer membrane proteins (porins). -Infect. Immun. 42, 219–223.
Wilton J.M.A. (1977): The function of complement in crevicular fluid. In the Borderland between Caries and Periodontal disease p. 223 Lehner T. (ed) Academic Press London.
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Tufano, M.A., Sommese, L. & Galdiero, F. Some biological activities of Eikenella corrodens major outer membrane proteins. Eur J Epidemiol 2, 305–311 (1986). https://doi.org/10.1007/BF00419495
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DOI: https://doi.org/10.1007/BF00419495