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Histamin in der Interstitialflüssigkeit der befallenen und klinisch nicht befallenen Haut bei chronisch rezidivierender Urticaria

Histamine levels in interstitial fluid of lesional and perilesional skin in patients with chronic idiopathic urticaria

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Summary

In 8 patients with idiopathic chronic urticaria suction blisters were produced simultaneously on lesional and perilesional skin in the periumbilical area without application of heat. The suction pressure was 250 mm Hg, and thus the blister production lasted from 60–120 min. The blister fluid histamine was determined with a modified autoanalyzer technique developed by Siraganian. In this method the extraction of histamine, the condensation of o-phthaldialdehyde with histamine and the measurement of the fluorochrome is automated. With the described method it is possible to measure values less than 0.5 ng/ml.

Histamine levels in perilesional skin were nearly in the same range as in the periumbilical skin of 10 normal persons. The range of the normal tissue fluid histamine levels was 0–15 ng/ml and they did not differ significantly from the plasma levels. In all 8 patients with chronic urticaria the histamine values were higher in lesional than in perilesional skin. In 1 patient the lesional blister fluid histamine value reached 35.5 ng/ml. Four of the patients showed only low differences (less than 3 ng/ml).

Zusammenfassung

Bei 8 Patienten mit idiopathischer chronisch rezidivierender Urticaria wurden in der Periumbilicalregion Saugblasen gleichzeitig auf frischen urticariellen Herden und der unveränderten Haut daneben produziert und Histamin in der Blasenflüssigkeit bestimmt. Hierzu wurde die von Siraganian entwickelte Methode der Autoanalyzer-Technik in modifizierter Form verwendet. Die Histaminspiegel lagen bei diesen Patienten in unveränderter Haut in derselben Größenordnung wie bei 10 Normalpersonen, während alle 8 Urticaria-Patienten höhere Histamin-Werte in den Urticae verglichen mit der nicht befallenen Haut aufwiesen; bei 4 der Patienten waren diese Unterschiede jedoch nur gering ausgeprägt.

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Authors and Affiliations

  1. Hautklinik (Direktor: Prof. Dr. G. W. Korting) der Johannes Gutenberg-Universität Mainz, Langenbeckstr. 1, D-6500, Mainz, Bundesrepublik Deutschland

    K. Bork & G. Böhm

  2. Institut für Medizinische Mikrobiologie (Direktor: Prof. Dr. P. Klein) der Johannes Gutenberg-Universität Mainz, Langenbeckstr. 1, D-6500, Mainz, Bundesrepublik Deutschland

    W. König

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  1. K. Bork
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  2. W. König
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  3. G. Böhm
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Herrn Prof. Dr. G. W. Korting zum 60. Geburtstag gewidmet

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Bork, K., König, W. & Böhm, G. Histamin in der Interstitialflüssigkeit der befallenen und klinisch nicht befallenen Haut bei chronisch rezidivierender Urticaria. Arch Dermatol Res 266, 269–276 (1979). https://doi.org/10.1007/BF00418572

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