Summary
Lactate dehydrogenase (LDH) isozymes in the epidermis and in transplantable epidermal carcinoma from Syrian hamsters were studied by polyacrilamide electrophoresis. A 5-band pattern with occasional loss of 1–2 anode fractions in certain tumor samples is typical. In the epidermis of 14 to 15-day-old foetuses, in the regenerating epidermis, and in tumor cells cathode or M subunits predominate, in particular the fifth fraction in the last two cases. In the epidermis from new born, 25-day-old (growing stage of the hairs) and 60 to 80-day-old (resting stage of the hairs) animals a relative increase of the H subunits is striking.
These results reveal a foetal-like pattern of LDH isozymes in regenerating and tumorous epidermis, as has been observed in other isozymes and in other tissues.
Zusammenfassung
Lactat-Dehydrogenase (LDH) Isoenzyme wurden in der Epidermis und in transplantablen epidermalen Carcinomen von syrischen Hamstern mittels Polyacrylamid-Elektrophorese untersucht. Im typischen Fall treten 5 Banden auf, wobei gelegentlich 1–2 Anodenfraktionen in bestimmten Tumorproben fehlen. In der Epidermis von 14–15 Tage alten Foeten, in regenerierender Epidermis und in Tumorzellen überwiegen die Kathoden oder M-Fraktionen, insbesondere in der 5. Fraktion der letzten beiden Fälle. In der Epidermis von Neugeborenen, 25 Tage alten (Haarwachstumsphase) und 60–80 Tage alten (Ruhephase des Haares) Tieren wird eine relative Zunahme der H-Fraktion beobachtet.
Diese Ergebnisse weisen auf eine Verteilung der LDH-Isoenzyme sowohl in regenerierender als auch in der Tumorepidermis wie bei Foeten hin, wie dies auch bei anderen Isoenzymen in anderen Geweben beobachtet wurde.
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Nicolov, I.G., Chernozemsky, I.N. & Petkova-Bocharova, T. LDH isozymes in epidermis and skin carcinoma in hamsters. Arch Dermatol Res 266, 233–238 (1979). https://doi.org/10.1007/BF00418569
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DOI: https://doi.org/10.1007/BF00418569
Key words
- LDH isozymes
- Epidermis
- Ontogenetic differences and regeneration
- Transplantable squamous cell carcinoma
- Syrian hamsters