Abstract
A mouse 7S RNA cDNA plasmid clone was employed to identify and map DNA restriction fragment variants using recombinant inbred (RI) and congenic mouse strains. More than a dozen such restriction variants were identified and mapped to different regions of the mouse genome. One such variant, designated Rn7s-6, showed close linkage to the Ly-2,3-Igk-V (T lymphocyte antigens 2 and 3, kappa immunoglobulin variable region) cluster of markers on chromosome 6. No recombinants were detected among three of these markers in 59 RI strains. On the basis of these data, the Rn7s-6 sequence may be placed within 1.3 centimorgans of Ly-3 and one of the Igk-V-region markers, Igk-Efl. Two mouse stocks with previously identified crossovers within the Ly2,3-Igk-V region were used to sublocalize Rn7s-6. The results are consistent with the gene order (Ly-2, Ly-3)-(Rn7s-6, Igk-Efl)-Igk-Ef2. Several mouse plasmacytomas, known to have various parts of the kappa chain complex deleted, retain the Rn7s-6 sequence. The Rn7s-6 variant is a plus/minus variant; no sequence allelic to Rn7s-6 is found in inbred strains that share the Ly-3 a-Igk-Efla haplotype.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alt, F. W., Enea, V., Bothwell, A. L. M., and Baltimore, D.: Activity of multiple light chain genes in murine myeloma cells producing a single functional light chain. Cell 21: 1–12, 1980
Balmain, A., Krumlauf, R., Vass, J. K., and Birnie, G. D.: Cloning and characterization of the abundant cytoplasmic 7S RNA from mouse cells. Nucleic Acids Res. 10: 4259–4277, 1982
Chung, S.-Y., Folsom, V., and Wooley, J.: DNase 1-hypersensitive sites in the chromatin of immunoglobulin x light chain genes. Proc. Natl. Acad. Sci. U.S.A. 80: 2427–2431, 1983
Church, G.M. and Gilbert, W.: Genomic sequencing. Proc. Natl. Acad. Sci. U.S.A. 81: 1991–1995,1984
Claflin, J. L., Taylor, B. A., Cherry M., and Cubberly, M.: Linkage in mice of genes controlling an immunoglobulin in kappa-chain marker and the surface alloantigen Ly-3 on T lymphocytes. Immunogenetics 6: 379–387, 1978
Cory, S., Tyler, B. M., and Adams, J. M.: Sets of immunoglobulin Vκ genes homologous to ten cloned Vκ sequences: Implications for the number of germline Vκ genes. J. Mol. Appl. Genet. 1: 103–116, 1981
Datta, S. K., Owen, F. L., Womack, J. E., and Riblet, R. J.: Analysis of recombinant inbred lines derived from “autoimmune” (NZB) and “high leukemia” (C58) strains: Independent multigenic systems control B cell hyperactivity, retrovirus expression, and autoimmunity. J. Immunol. 129: 1539–1544, 1982
Durdik, J., Moore, M. W., and Selsing, E.: Novel K light-chain gene rearrangements in mouse λ light chain-producing B lymphocytes. Nature 307: 749–752, 1984
Erikson, E., Erikson, R. L., Henry, B., and Pace, N. R.: Comparison of oligonucleotides produced by RNase T1 digestion of 7/S RNA from avian and murine oncornaviruses and from uninfected cells. Virology 53: 40–46, 1973
Gibson, D. M., Taylor, B. A., and Cherry, M.: Evidence for close linkage of a mouse light chain marker with the Ly-2,3 locus. J. Immunol. 121: 1585–1590, 1978
Gibson, D. M., MacLean, S. J., and Cherry, M.: Recombination between kappa chain genetic markers and the Lyt-3 locus. Immunogenetics 18: 111–116, 1983
Gibson, D. M., MacLean, S. J., Anctil, D., and Mathieson, B. J.: Recombination between kappa chain genetic markers in the mouse. Immunogenetics 20: 493–501, 1984
Gottlieb, P. D., Marshak-Rothstein, A., Auditore-Hargreaves, K., Berkoben, D. B., August, D. A., and Rosche, R. M.: Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and antiLyt-3.1 monoclonal antibodies. Immunogenetics 10: 545–555, 1980
Gottlieb, P. D., Tsang, H. C. W., Gibson, D. M., and Cannon, L. E.: Unique Vκ group associated with two mouse L chain genetic markers. Proc. Natl. Acad. Sci. U.S.A. 78: 559–563, 1981
Itakura, K., Hutton, J. J., Boyse, E. A., and Old, L. J.: Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse. Transplantation 13: 239–243, 1972
Lazure, C., Hum, W. T., and Gibson, D. M.: A major group of mouse kappa chains controlled by the chromosome 6 locus, Igk-Ef2. J. Exp. Med. 152: 555–564, 1980
Li, W.-Y., Reddy, R., Henning, D., Epstein, P., and Busch, H.: Nucleotide sequences of 7S RNA. Homology to Alu and La 4.5 S RNA. J. Biol. Chem. 257: 5136–5142, 1982
Mather, E. L. and Perry, R. P.: Methylation status and DNase I sensitivity of immunoglobulin genes: Changes associated with rearrangement. Proc. Natl. Acad. Sci. U.S.A. 80: 4689–4693, 1983
Moynet, D., MacLean, S. J., Ng, K. H., Anctil, D., and Gibson, D. M.: Polymorphism of κ variable region (Vκ-1) genes in inbred mice: Relationship to the Igk-Ef2 serum light chain marker. J. Immunol. 135: 727–732, 1985
Riblet, R., Claflin, L., Gibson, D. M., Mathieson, B. J., and Weigert, M.: Antibody gene linkage studies in (NZB x C58) recombinant inbred lines. J. Immunol. 124: 787–789, 1980
Seidman, J. G., Nau, M. M., Norman, B., Kwan, S.-P., Scharff, M., and Leder, P.: Immunoglobulin V/J recombination is accompanied by deletion of joining site and variable region segments. Proc. Natl. Acad. Sci. U.S.A. 77: 6022–6026, 1980
Selsing, E. and Storb, U.: Mapping of immunoglobulin variable region genes: Relationship to the ‘deletion’ model of immunoglobulin gene rearrangement. Nucleic Acids Res. 9: 5725–5735, 1981
Stevens, L. C. and Varnum, D. S.: The development of teratomas from parthenogenetically activated ovarian mouse eggs. Dev. Biol. 37: 369–380, 1974
Taylor, B. A. and Rowe, L.: Genes for serum amyloid A proteins map to chromosome 7 in the mouse. Mol. Gen. Genet. 195: 491–499, 1984
Tulchin, N. and Taylor, B. A.: γ-glutamyl cyclotransferase: A new genetic polymorphism in the mouse (Mus musculus) linked to Lyt-2. Genetics 99: 109–116, 1981
Ullu, E. and Melli, M.: Cloning and characterization of cDNA copies of the 7S RNA of HeLa cells. Nucleic Acids Res. 10: 2209–2223, 1982
Ullu, E. and Weiner, A.M.: Human genes and pseudogenes for-the 7SL RNA component of signal recognition particle. EMBO J. 3: 3303–3310, 1984
Ullu, E., Murphy, S., and Melli, M.: Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an Alu sequence. Cell 29: 195–202, 1982
Van Ness, B. G., Coleclough, C., Perry, R. P., and Weigert, M.: DNA between variable and joining gene segments of immunoglobulin κ light chain is frequently retained in cells that rearrange the x locus. Proc. Natl. Acad. Sci. U.S.A. 79: 262–266, 1982
Walter, P. and Blobel, G.: Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum. Nature 299: 691–698, 1982
Wilson, R. and Storb, U.: Association of two different repetitive DNA elements near immunoglobulin light chain genes. Nucleic Acids Res. 11: 1803–1817, 1983
Yetter, R. A., Hartley, J. W., and Morse III, H. C.: H-2 linked regulation of xenotropic murine leukemia virus expression. Proc. Natl. Acad. Sci. U.S.A. 80: 505–509, 1983
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, B.A., Rowe, L., Gibson, D.M. et al. Linkage of a 7S RNA sequence and kappa light chain genes in the mouse. Immunogenetics 22, 471–481 (1985). https://doi.org/10.1007/BF00418092
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00418092