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Fibrilläre und tubuläre Feinstrukturen im Cytoplasma von Entamoeba histolytica

Fibrillar and tubular fine structures in the cytoplasm of Entamoeba histolytica

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Summary

The electron-microscopical study of conventional fixed and contrasted Entamoeba histolytica-trophozoites from Diamonds monoxenic TTY-medium revealed “thick fibrils” in the vesicular cytoplasm of the parasites.

They are 9–14 nm in diameter and are therefore considered to be myosin-like filaments, that are spread mainly in the tail or the uroid of the moving ameba. No association with microfilaments (MF) or other organelles could be observed.

During the same investigation microtubules (MT) of variable length are described having a diameter of 35 nm, surrounded by a 19–25 nm wide capsule. They are distributed singly or in bundles with a maximal diameter of 350 nm. Some of the MT were coiled up to a helical shape.

Zusammenfassung

Im Cytoplasma intakter Kulturformen von zwei Entamoeba histolytica-Stämmen aus dem monoxenischen TTY-Medium nach Diamond wurden myosinähnliche Fibrillen in situ mit einem Durchmesser von 9–14 nm beobachtet.

Diese „dicken Fibrillen“ wurden vor allem im Hinterende der Amöben, die während aktiver Fortbewegung fixiert wurden, angetroffen, wo sie meist einzeln im Plasma zerstreut vorkommen. Eine Beziehung zu Mikrofilamenten vom Actintyp oder anderen Zellorganellen konnte nicht festgestellt werden.

Ebenfalls wurden bei den gleichen Trophozoiten Mikrotubuli mit einem Durchmesser von 35 nm beschrieben, die durch eine Hülle mit variablem Durchmesser von 19–25 nm umgeben sind. Die MT liegen einzeln oder in größeren Bündeln mit einem Durchmesser bis zu 350 nm unregelmäßig zerstreut zu Gruppen vereinigt in Cytoplasma. Als besondere Differenzierung werden spiralig gewundene MT dargestellt.

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Michel, R., Schupp, E. Fibrilläre und tubuläre Feinstrukturen im Cytoplasma von Entamoeba histolytica . Z. F. Parasitenkunde 47, 11–21 (1975). https://doi.org/10.1007/BF00418061

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