Skip to main content
SpringerLink
Log in

Chemical induction of quadruple and octuple chromosomes in Chinese hamster CHO-K1 cells and relationship between their three-dimensional structure and spatial distribution of BrdU-labeled chromatids

  • Original Articles
  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

Double endoreduplication of Chinese hamster CHO-K1 cells that exhibited quadruple chromosomes at metaphase was induced by a combination of rotenone and ammonium vanadate treatments. Analysis of sister chromatid differential staining patterns (using 5-bromo-2′-deoxyuridine) revealed that approximately 50% of the quadruple chromosomes did not keep the scheme of “outside replication” of DNA. Based on the ratio of the staining patterns observed, we suggest that the two diplochromosomes forming a quadruple chromosome are held together by a physical link connecting the two original chromatids. Metaphases with octuple chromosomes were also produced by the same treatment. Each chromosome constituting an octuple chromosome was longer and thinner than ordinary metaphase chromosmes. This suggests incomplete chromosome condensation at metaphase. The majority of octuple chromosomes showed the eight constituent chromosomes to be so enmeshed that a planar alignment could not be observed in air-dried preparations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Goyanes VJ, Schvartzman JB (1981) Insights on diplochromosome structure and behaviour. Chromosoma 83:93–102

    Google Scholar 

  • Herreros B, Giannelli F (1967) Spatial distribution of old and new chromatid sub-units and frequency of chromatid exchanges in induced human lymphocyte endoreduplication. Nature 216:286–288

    Google Scholar 

  • Matsumoto K, Ohta T (1992) Sensitive period for the induction of endoreduplication by rotenone in cultured Chinese hamster cells. Chromosoma 102:60–65

    Google Scholar 

  • Matsumoto K, Ohta T (1993) Mitosis of rotenone-induced endore-duplication in Chinese hamster cells. Jpn J Genet 68:185–194

    Google Scholar 

  • Owusu-Yaw J, Cohen MD, Fernando SY, Wei CI (1990) An assessment of the genotoxicity of vanadium. Toxicol Lett 50:327–336

    Google Scholar 

  • Rizzoni M, Palitti F (1973) Regulatory mechanism of cell division, I. Colchicine-induced endoreduplication. Exp Cell Res 77:450–458

    Google Scholar 

  • Ronchi VN, Avanzi S, D'Amato F (1965) Chromosome endoreduplication (endopolyploidy) in pea root meristems induced by 8-azaguanine. Caryologia 18:599–617

    Google Scholar 

  • Schwarzacher HG, Schnedl W (1965) Endoreduplication in human fibroblast cultures. Cytogenetics 4:1–18

    Google Scholar 

  • Schwarzacher HG, Schnedl W (1966) Position of labelled chromatids in diplochromosomes of endoreduplicated cells after uptake of tritiated thymidine. Nature 209:107–108

    Google Scholar 

  • Speit G, Mehnert K, Vogel W (1984) Induction of endoreduplication by hydrazine in Chinese hamster V79 cells and reduced incidence of sister chromatid exchanges in endoreduplicated mitoses. Chromosoma 89:79–84

    Google Scholar 

  • Sutou S, Tokuyama F (1974) Induction of endoreduplication in cultured mammalian cells by some chemical mutagens. Cancer Res 34:2615–2623

    Google Scholar 

  • Takanari H (1985) Studies on endoreduplication, V. A three-dimensional scheme for diplo- and quadruple chromosomes and a model for DNA replication. Cytogenet Cell Genet 39:188–193

    Google Scholar 

  • Takanari H, Nakakuki K, Izutsu K (1985) Cytogenetic demonstration of out-of-phase DNA synthesis in endoreduplicated CHO cells: evidence for partial endoreduplication. Cytogenet Cell Genet 39:93–98

    Google Scholar 

  • Takayama S, Tachibana K (1980) Two opposite types of sister chromatid differential staining in BUdR-substituted chromosomes using tetrasodium salt of EDTA. Exp Cell Res 126:498–501

    Google Scholar 

  • Takayama S, Hirano Y, Hiramatsu H (1992) Induction of endoreduplication in double minutes of the human neuroblastoma cells and the replication pattern. Jpn J Genet 67:397–403

    Google Scholar 

  • Walen KH (1965) Spatial relationships in the replication of chromosomal DNA. Genetics 51:915–929

    Google Scholar 

  • Wolf S, Perry P (1974) Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma 48:341–353

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Genetic Toxicology, Institute of Environmental Toxicology, Suzuki-cho 2-772, 187, Kodaira, Tokyo, Japan

    Kyomu Matsumoto & Toshihiro Ohta

Authors
  1. Kyomu Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toshihiro Ohta
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsumoto, K., Ohta, T. Chemical induction of quadruple and octuple chromosomes in Chinese hamster CHO-K1 cells and relationship between their three-dimensional structure and spatial distribution of BrdU-labeled chromatids. Chromosoma 103, 338–342 (1994). https://doi.org/10.1007/BF00417881

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00417881

Keywords

Search

Navigation