Abstract
Using fluorescence in situ hybridization (FISH) with probe pScT7, three different 5S rDNA loci were detected in the satellite of rye chromosome 1R (5SDna-R1) and in the short arms of chromosomes 3R (5SDna-R3) and 5R (5SDna-R2) respectively. All three loci showed polymorphism for the hybridization signal intensity. In order to determine the localization of these rye 5S rDNA multigene loci with higher precision within the corresponding chromosome arms, the probe pScT7 was physically mapped by FISH in relation to the following five translocations (Wageningen Tester Set): T850W (1RS/4RL), T248W (1RS/6RS), T273W (1RS/5RL), T305W (2RS/5RS) and T240W (3RS/5RL). Accurate physical maps of the translocation breakpoints had previously been made using electron microscope analysis of spread pachytene synaptonemal complexes of heterozygotes for the different translocations. The results indicate that locus 5SDna-R3 is located between the breakpoint of translocation T240W and the telomere, whereas locus 5SDna-R2 is located between the breakpoint of translocation T305W and the centromere, the hybridization of probe pScT7 on T305W translocated chromosomes demonstrating the complex nature of this translocation. On the other hand, the simultaneous detection of probes pScT7 and pTA71 (18S-5.8S-26S rDNA) with two different fluorochromes, indicated that the breakpoints of translocations T850W and T248W are located between loci Nor-R1 and 5SDna-R1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alonso-Blanco C, Goicoechea PG, Roca A, Giraldez R (1993) A cytogenetic map of the entire length of rye chromosome 1R, including one translocation breakpoint, three isozyme loci and four C-bands. Theor Appl Genet 85:735–744
Appels R, Moran B (1984) Molecular analysis of alien chromatin introduced into wheat. In: Gustafson JP (ed) Gene manipulation in plant improvement (16th Stadler Genetics Symposium). Plenum Press, New York, pp 529–557
Appels R, Gerlach WL, Dennis ES, Swift H, Peacock WJ (1980) Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma 78:293–311
Alvarez E, Alonso-Blanco C, Roca A, Goicoechea PG, Giraldez R (1994) Physical mapping of translocation breakpoints in rye by means of synaptonemal complex analysis. Theor Appl Genet (in press)
Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560
Clark M, Karp A, Archer S (1989) Physical mapping of the B-hordein loic on barley chromosome 5 by in situ hybridization. Genome 32:925–929
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1855
Giraldez R, Cermeño MC, Orellana J (1979) Comparison of C banding pattern in the chromosomes of inbred lines and open pollinated varieties of rye. Z Pflanzenzuecht 83:40–48
Gustafson JP, Butler E, McIntyre L (1990) Physical mapping of at low-copy DNA sequence in rye (Secale cereale L.). Proc Natl Acad Sci USA 87:1899–1902
Heng HHQ, Tsui L (1993) Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma 102:325–332
Jones JDG, Flavell RB (1982a) The structure and chromosomal localisation of defined repeated DNA sequences in species of the genus Secale. Chromosoma 86:613–641
Jones JDG, Flavell RB (1982b) The mapping of highly-repeated DNA families and their relationship to C-bands in chromosomes of Secale cereale. Chromosoma 86:595–612
Jong JH de, van Eden J, Sybenga J (1989) Synaptonemal complex formation and metaphase I configuration patterns in a translocation heterozygote of rye (Secale cereale). Genome 32:72–81
Korenberg JR, Yang-Feng T, Schreck R, Chen XN (1992) Using fluorescence in situ hybridization (FISH) in genome mapping. TIBTECH 10:27–32
Kota RS, Gill KS, Gill BS, Endo TR (1993) A cytogenetically based physical map of chromosome 1B in common wheat. Genome 36:548–554
Lagudah ES, Appels R, Brown ADH, McNeil D (1991) The molecular genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386
Lawrence GJ, Appels R (1986) Mapping the nucleolus organizer region, seed protein loci and isozyme loci on chromosome 1R in rye. Theor Appl Genet 71:742–749
Lawrence JB (1990) A fluorescence in situ hybridization approach for mapping and the study of nuclear organization. In: Davies KE, Tilghman SM (eds) Genetic and physical mapping Cold Spring Harbor Laboratory Press, pp 1–38
Lehfer H, Busch W, Martin R, Herrmann RG (1993) Localization of the B-hordein locus on barley chromosomes using fluorescence in situ hybridization. Chromosoma 102:428–432
Leitch IJ, Heslop-Harrison JS (1992) Physical mapping of the 18S-5.8S-26S rRNA genes in barley by in situ hybridization. Genome 35:1013–1018
Leitch IJ, Heslop-Harrison JS (1993) Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare). Genome 36:517–523
Miller TE, Hutchinson J, Reader SM (1983) The identification of the nucleolus organiser chromosomes of diploid wheat. Theor Appl Genet 65:145–147
Mukai Y, Endo TR, Gill BS (1991) Physical mapping of the 18S.26S rRNA multigene family in common wheat: identification of a new locus. Chromosoma 100:71–78
Naranjo T, Roca A, Goicoechea PG, Jong JH de, Smilde WD (1989) Comparison of synaptonemal complex at pachytene and chromosome association at metaphase I in heterozygotes for a reciprocal translocation of rye. Genome 32:983–991
Pendás AM, Morán P, García-Vázquez E (1993a) Ribosomal RNA genes are interspersed throughout a heterochromatic chromosome arm in Atlantic salmon. Cytogenet Cell Genet 63:128–130
Pendás AM, Morán P, García-Vázquez E (1993b) Multichromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chromosome Res 1:63–67
Pinkel D, Straume T, Gray JW (1986) Cytogentic analysis using quantitative high sensitivity fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938
Reddy P, Appels R (1989) A second locus for the 5S multigene family in Secale L.: sequence divergence in two lineages of the family. Genome 32:456–467
Rogowsky PM, Sorrels ME, Shepherd KW, Langridge P (1993) Characterisation of wheat-rye recombinants with RELP and PCR probes. Theor Appl Genet 85:1023–1028
Schwarzacher T, Heslop-Harrison JS (1991) In situ hybridization to plant telomeres using synthetic oligomers. Genome 34:317–323
Sybenga J, Wolters HG (1972) The classification of the chromosomes of rye (Secale cereale L.): a translocation tester set. Genetica 43:453–464
Sybenga J, Parmar S, van Eden J, Shewry P (1990) Mapping seed storage protein loci Sec-1 and Sec-3 in relation to five chromosomal rearrangements in rye (Secale cereale L.). Theor Appl Genet 79:201–208
de Vries JM, Sybenga J (1976) Identification of rye chromosomes: the Giemsa banding pattern and the Translocation Tester Set. Theor Appl Genet 48:35–43
Wiegant J, Ried T, Nederlof PM, Van der Ploeg M, Tanle HJ, Raap AK (1991) In situ hybridisation with fluoresceinated DNA. Nucleic Acids Res 19:3277–3241
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alonso-Blanco, C., Pendás, A.M., Garcia-Suarez, R. et al. Physical mapping of 5S rDNA reveals a new locus on 3R and unexpected complexity in a rye translocation used in chromosome mapping. Chromosoma 103, 331–337 (1994). https://doi.org/10.1007/BF00417880
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00417880