Summary
Samples from the L-428 Hodgkin's cell line and from several other lymphoma cell lines were lysed and the soluble proteins were subjected to one-or two-dimensional gel electrophoresis. The separated proteins were transferred to nitrocellulose by the Western Blot technique; antibody reactivity was detected by an immunoperoxidase reaction. Of 152 sera from patients with Hodgkin's disease (HD) 26 (17%) reacted with protein P-65 whereas in the control group, consisting of 35 healthy persons and 20 patients with other malignant diseases only 1 serum was reactive. Thus, the difference between the two groups was highly significant (P<0.01). These antibodies were most common in stage III HD. Splenectomy had no effect on the incidence of these antibodies, and there seemed to be no correlation with B-symptoms or with the histological subtype.
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Dedicated to Professor Dr. G. W. Löhr on the occasion of this 65th birthday
Supported by the Deutsche Forschungsgemeinschaft
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Hirsch, F.W., Scholl, J. & Dölken, G. Serum antibodies to a normal cellular protein (P-65) in patients with Hodgkin's disease. J Cancer Res Clin Oncol 114, 204–207 (1988). https://doi.org/10.1007/BF00417838
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DOI: https://doi.org/10.1007/BF00417838