Summary
An N-ethyl-N-nitrosourea-induced rat ovarian Sertoli cell tumor was grown in tissue culture in Dulbecco's modified Eagle's medium (DMEM) supplemented with 25% horse serum (HS) and a hormone combination of 20 ng/ml each of hydrocortisone, insulin, and prolactin. This tissue culture derived from a nonsteroid hormone-producing tumor. Cytofluorometry and karyotyping of the nonhormone-producing tumor cell line (SCTL-1) revealed a diploid pattern for the early passage (P1), which became hyperdiploid (P10), and then aneuploid (P20). These cells had an epitheloid pattern, grew in a monolayer at early passages. After P10 the cells were transplanted into newborn rats and nude mice and resulted in high incidences of tumors (up to 100%). The cell line (SCTL-1) continued to grow in DMEM, 10% HS, and no hormone supplementation after P10. This study revealed that a benign rat ovarian Sertoli cell tumor after multiple passages in vitro underwent sequential genotypic and phenotypic changes and became highly malignant.
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References
Ashley DJB (1978) In: Evan's histological appearances of tumors, 3rd edn. Churchill, Livingston, Edinburgh London New York, pp 663–672
Cotchin E (1977) In: Blaustein (ed) Pathology of the female genital tract. Springer, Berlin Heidelberg New York, pp 836–838
D'Ambrosio SM, Su C, Chang MJW, Oravec C, Stoica G, Koestner A (1986) DNA damage, repair, replication and tumor incidence in the BD-IV rat strain following administration of N-ethyl-N-nitrosourea. Anticancer Res 6:49–54
Druckrey H, Landschultz CH, Ivankovic S (1970) Transplacentare Erzeugung maligner Tumoren des Nervensystems. II. Athylnitrosoharnstoff an 10 genetisch definierten Rattenstämmen. Z Krebsforsch 73:371–386
Engel LL (1960) Recent studies on estrogen metabolism. In: Pincul, Vollmer (eds) Biological activities of steroids in relation to cancer. Academic Press, New York, p III
Koestner A, Swenberg JA, Weschler W (1972) Experimental tumors of the nervous system induced by resorptive N-nitrosourea compounds. Rec Adv Brain Tumor Res Hamburg Fed 7:9–30
Krishan A (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodine staining. J Cell Biol 66:188–193
Mac Pherson (1972) Soft agar techniques. In: Kruse, Patterson (eds) Tissue culture methods and applications. Academic press, New York, pp 276–280
Maekawa A, Onodera H, Tonigawa H, Kanno J, Furuta K, Hayashi Y (1986) Induction of Sertoli cell tumor in the rat ovary by N-alkyl-N-nitrosoureas. Cancer Res Clin Oncol 111(2):173–176
Meyer R (1931) The pathology of some special ovarian tumors and their relation with sex characteristics. Am J Obstet Gynecol 22:691–713
Pastan IH (1973) Cell culture. In: Jakoby (ed) Methods in enzymology, vol LVIII, Academic Press, New York, p 150
Prestone-Martin S, Yu CM, Benton B, Henderson ER (1982) N-Nitroso compounds and childhood brain tumors: a case-control study. Cancer Res 42:5240–5245
Rajewsky MF, Augenlicht LH, Biessmann H, Goth R, Husler DF, Laerum OD, Lomakina LY (1977) Nervous-systemic-specific carcinogenesis by ethylnitrosourea in the rat: molecular and cellular aspects. In: Hiatt HH, Watson JD, winsten JA (eds) Cold Spring Harbor, New York, pp 709–726
Stoica G, Koestner A (1984) Diverse spectrum of tumors in male Sprague-Dawley rats following single high doses of N-ethyl-N-nitrosourea (ENU). Am J Pathol 116:319–326
Stoica G, Koestner A, Capen CC (1985) Testicular (Sertoli's cell)-like tumors of the ovary induced by N-ethyl-N-nitrosourea (ENU) in rats. Vet Pathol 22:483–491
Stoica G, Capen C, Koestner A (1987) Sertoli's cell tumor, ovary, rat. In: Jones TC, Mohr U, Hunt RD (eds) Monographs on pathology of laboratory animals. Genital system. Springer, New York, pp 30–36
Tracy SI, Askin FB, Reddick RL, Jackson B, Kurman RJ (1985) Progesterone secreting Sertoli cell tumor of the ovary. Gynecol Oncol 22:85–96
Unakul W, Hsu TC (1972) The C-and G-banding patterns of Rattus norvegicus chromosomes. J Natl Cancer Inst 49:1425–1431
Yunis JJ (1981) New chromosome techniques in the studies of human neoplasia. Hum Pathol 12:540–549
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Stoica, G., O'Leary, M. In vitro malignant transformation of in vivo ENU-induced rat ovarian Sertoli cell tumor (adenoma). J Cancer Res Clin Oncol 114, 142–148 (1988). https://doi.org/10.1007/BF00417828
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DOI: https://doi.org/10.1007/BF00417828