Skip to main content
SpringerLink
Log in

A study of light-induced conductivity in bacteriorhodopsin

  • Published:
Journal of Biological Physics Aims and scope Submit manuscript

Abstract

Measurements have been made of light-induced conductivity changes and the associated kinetics of the relaxation processes in aqueous suspensions and sonicated liposomes containing bacteriorhodopsin (bR). Aqueous suspensions exhibit a single relaxation time of 1 to 2 ms. The addition of D2O to the aqueous suspension slows down the relaxation time, fourfold. Similar behaviour is seen in sonicated liposomes with a relaxation time of 2 to 3 ms. Activation energies of approximately 14 and 6 kJM-1 are obtained for the effect in sonicated liposomes and aqueous suspension containing bR, respectively. These relaxation processes with lifetime of 1 to 2 ms suggest conformational changes in the protein moiety of bR which most probably may be associated with protonation-deprotonation processes or less likely the release and binding of small ions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahl, P. L. and Cone, R. A., 1984, Biophys. J. 45, 1039–1049.

    Google Scholar 

  • Anderson, W. A. and Arnold, J. T., 1956, Phys. Rev. 101, 511–512.

    Google Scholar 

  • Austin, R. H., Beeson, K. W., Eisenstein, L., Frauenfelder, H., and Gunsalus, I. C., 1975, Biochemistry 14, 5355–5373.

    Google Scholar 

  • Becher, B., Tokunaga, F., and Ebrey, T. G., 1978, Biochemistry 17, 2293–2300.

    Google Scholar 

  • Beece, D., Bowne, S. F., Czege, J., Eistenstein, L., Frauenfelder, H., Good, D., Marden, M. C., Marque, J., Ormos, P., Reinisch, L., and Yue, K. T., 1981, Photochem. Photobiol. 33, 517–522.

    Google Scholar 

  • Birge, R. R. and Copper, T. M., 1983, Biophys. J. 42, 61–69.

    Google Scholar 

  • Blaurock, A. E., 1975, J. Mol. Biol. 93, 139–158.

    Google Scholar 

  • Bogomolni, R. A., Stubbs, L., and Lanyi, J. K., 1978, Biochemistry 17, 1037–1041.

    Google Scholar 

  • Bolton, P. D. and Hefler, L. G., 1971, Quart. Rev. 25, 521–525.

    Google Scholar 

  • Brainman, M. and Mathies, R., 1980, Biochemistry 19, 5421–5428.

    Google Scholar 

  • Braunstein, D., Kyung, M. K., Lin, S. L., Ormos, P., and Vittitow, J., 1987, Biophys. J. 51, 135a.

    Google Scholar 

  • Camelli, c., Quintanilha, A. T., Alexandre, T., and Parker, L., 1980, Proc. Natl. Acad. Sci. USA 77, 4707–4711.

    Google Scholar 

  • Dencher, N. A. and Wilms, M., 1975, Biophys. Struct. Mech. 1, 259–271.

    Google Scholar 

  • Dencher, N. A., Dresselhaus, D., Zaccai, G., and Buldt, G., 1989, Proc. Natl. Acad. Sci. USA 86, 7876–7879.

    Google Scholar 

  • Dewey, T. G., 1987, Biophys. J. 51, 809–815.

    Google Scholar 

  • Dewey, T. g. and Hammes, G. G., 1981, Proc. Natl. Acad. Sci. USA 78, 7422–7425.

    Google Scholar 

  • Doster, W., 1983, Biophys. Chem. 17, 97–103.

    Google Scholar 

  • Draheim, J. E. and Cassim, J. Y., 1985, Biophys. J. 47, 497–508.

    Google Scholar 

  • Ebrey, T. G., 1982, in A. Martonosi (ed.), Membrane and Transport, Plenum, New York, pp. 323–328.

    Google Scholar 

  • Fukumoto, J. M., Hanamoto, J. H., and El-Sayed, M. A., 1984, Photochem. Photobiol. 39, 75–79.

    Google Scholar 

  • Glaeser, R. M., Baldwin, J., Ceska, T. A., and Henderson, R., 1986, Biophys. J. 50, 913–920.

    Google Scholar 

  • Grzesiek, S. and Dencher, N. A., 1986, FEBS Lett. 208, 337–342.

    Google Scholar 

  • Hasselbacher, C. A., Preuss, D., and Dewey, T. G., 1986a, Biochemistry 25, 668–676.

    Google Scholar 

  • Hasselbacher, C. A., and Dewey, T. G., 1986b, Biochemistry 25, 6236–6243.

    Google Scholar 

  • Henderson, R., 1975, J. Mol. Biol. 92, 123–138.

    Google Scholar 

  • Hess, B. and Kuschmitz, D., 1979, FEBS Lett. 100, 334–340.

    Google Scholar 

  • Holz, M., Lindau, M., and Heyn, M. P., 1991, Biophys. J. 53, 623–633.

    Google Scholar 

  • Hsieh, C-L., Nagumo, M., Nicol, M., and El-Sayed, M. A., 1981, J. Phys. Chem. 85, 2714–2717.

    Google Scholar 

  • Huang, J. Y. and Lewis, A., 1989, Biophys. J. 55, 835–842.

    Google Scholar 

  • Jahnig, E., 1981, Biophys. J. 36, 329–358.

    Google Scholar 

  • Kalisky, O., Ottolenghi, M., Honig, B., and Korenstein, R., 1981, Biochemistry 20, 649–655.

    Google Scholar 

  • Keszthelyi, L. and Ormos, P., 1980, FEBS Lett. 109, 189–193.

    Google Scholar 

  • Konishi, T. and Parker, L., 1978, FEBS Lett. 92, 1–4.

    Google Scholar 

  • Korenstein, R. and Hess, B., 1977, Nature (London) 270, 184–186.

    Google Scholar 

  • Kuschmitz, D. and Hess, B., 1981, Biochemistry 20, 5950–5957.

    Google Scholar 

  • Lazarev, Y. A. and Terpugov, E. T., 1980, Biochim. Biophys. Acta 590, 324–338.

    Google Scholar 

  • Lewis, A., Spoonhower, J., Bogomolni, R. A., Lozier, R. H., and Stoeckenius, W., 1974, Natl. Acad. Sci. USA 71, 4462–4466.

    Google Scholar 

  • Lin, S. W. and Mathies, R. A., 1989, Biophys. J. 56, 653–660.

    Google Scholar 

  • Liu, S. Y., 1990, Biophys. J. 57, 943–950.

    Google Scholar 

  • Liu, S. Y., Gouindjee, R., and Ebrey, T. G., 1990, Biophys. J. 57, 951–963.

    Google Scholar 

  • Lozier, R. H., Niederberger, W., Bogomolni, R. A., Hwang, S. B., and Stoekenius, W., 1979, Biochim. Biophys. Acta 440, 545–556.

    Google Scholar 

  • Marinetti, T. and Mauzerall, D., 1983, Proc. Natl. Acad. Sci. USA 80, 178–180.

    Google Scholar 

  • Marinetti, T., 1987a, FEBS Lett. 216, 155–158.

    Google Scholar 

  • Marinetti, T., 1987b, Biophys. J. 51, 875–881.

    Google Scholar 

  • Oesterhelt, D. and Stoeckenius, W., 1971, Nature (London), New Biol. 233, 149–152.

    Google Scholar 

  • Oesterhelt, D. and Hesse, B., 1973, Eur. J. Biochem. 37, 316–326.

    Google Scholar 

  • Oesterhelt, D. and Stoeckenius, W., 1974, Methods Enzymol. 31, 667–678.

    Google Scholar 

  • Pettei, M. J., Yudd, A. P., Nakanishi, K., Hanselman, R., and Stoeckenius, W., 1977, Biochemistry 16, 1955–1959.

    Google Scholar 

  • Racker, E., 1973, Biochem. Biophys. Res. Commun. 55, 224–230.

    Google Scholar 

  • Rafferty, C. N., 1979, Photochem. Photobiol. 29, 109–320.

    Google Scholar 

  • Rothschild, K. J., Zagaeski, M., and Cantore, W. A., 1981, Biochem. Biophys. Res. Commun. 103, 483–489.

    Google Scholar 

  • Siebert, F., Mantele, W., and Kreutz, K., 1982, FEBS Lett. 141, 82–87.

    Google Scholar 

  • Sinton, M. H. and Dewey, T. G., 1988, Biophys. J. 53, 153–162.

    Google Scholar 

  • Slifkin, M. A. and Caplan, S. R., 1975, Nature (London) 253, 56–58.

    Google Scholar 

  • Slifkin, M. A. and Darby, M. I., 1978, J. Phys. E: Sci. Instrum. 11, 1166–1168.

    Google Scholar 

  • Slifkin, M. A., Garty, H., Sherman, W. V., Vincent, M. F. P., and Caplan, S. R., 1979, Biophys. Struct. Mech. 5, 313–320.

    Google Scholar 

  • Slifkin, M. A. and Darby, M. I., 1980, J. Phy. E: Sci. Instrum. 13, 896–897.

    Google Scholar 

  • Slifkin, M. A. and Ali, S. M., 1984, J. Mol. Liquids 28, 215–221.

    Google Scholar 

  • Slifkin, M. A. and Ali, S. M., 1987, Electrochim. Acta 32, 563–576.

    Google Scholar 

  • Slifkin, M. A., Bakarudin, I., Bradley, S., and Caplan, S. R., 1988, J. Bioenerg. Biomembr. 20, 585–602.

    Google Scholar 

  • Slifkin, M. A. and Ali, S. M., 1991, J. Chem. Soc., Faraday Trans. 87, 3241–3243.

    Google Scholar 

  • Stoeckenius, W. and Bogomolni, R. A., 1982, Ann. Rev. Biochem. 52, 587–616.

    Google Scholar 

  • Trissl, H-W., 1990, Photochem. Photobiol. 51, 793–818.

    Google Scholar 

  • Tsuda, M., Glaccum, M., Nelson, B., and Ebrey, T. G., 1980, Nature (London) 287, 351–353.

    Google Scholar 

  • Tsuda, M., Govindjee, R., and Ebrey, T. G., 1983, Biophys. J. 44, 249–254.

    Google Scholar 

  • Unwin, P. N. T. and Henderson, R., 1975, J. Mat. Biol. 94, 425–440.

    Google Scholar 

  • Warshel, A. and Barboy, N., 1982, J. Am. Chem. Soc. 104, 1469–1476.

    Google Scholar 

  • Weber, G., 1977, J. Chem. Phys. 66, 4081–4091.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Physics, University of Science Malaysia, 11800 US, Penang, Malaysia

    I. Bakarudin

  2. Department of Electronic and Electrical Engineering, University of Salford, M5 4WT, Salford, U.K.

    M. A. Slifkin

Authors
  1. I. Bakarudin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Slifkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bakarudin, I., Slifkin, M.A. A study of light-induced conductivity in bacteriorhodopsin. J Biol Phys 18, 203–216 (1991). https://doi.org/10.1007/BF00417809

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00417809

Key words

Search

Navigation