Abstract
Elastic and inelastic components of neutron scattering from lyophilized myoglobin were measured at wavevector transfers, Q of 1.0, 1.4, 1.85, and 3.0 Å-1 and at temperatures of 77 and 298 K. The elastic scattering determines an overall effective mean square atomic displacement which was employed in a simple harmonic Langevin formalism to obtain effective force constants describing molecular deformations. These force constants and the inelastic data are compatible with alpha helices as a vibrating species at room temperature, and with amino acid residues as the vibrating entities at 77 K. The present measurements are consistent with recent molecular dynamics calculations.
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SwaminathanS., IchiyeT., vanGunsterenW., and KarplusM.: Biochemistry 21 (1982), 5230.
ElberR. and KarplusM.: Science 235 (1987), 318.
BrownK. G., ErfurthS. S., SmallE. W., and PeticolasW. L.: Proc. Natl. Acad. Sci. USA 69 (1972), 1497.
BrockhouseB. N.: Methods for neutron spectroscopy, in Inelastic Scattering of Neutrons in Solids and Liquids, International Atomic Energy Agency, Vienna, 1961, pp. 113–151.
SmithJ., CusackS., PezzecaU., BrooksB., and KarplusM.: J. Chem. Phys. 85 (1986), 3636.
HartmannH., ParakF., SteigemannW., PetskoG. A., PonziD. R., and FrauenfelderF.: Proc. Natl. Acad. Sci. 79 (1982), 4967.
ParakF., KnappE. W., and KucheidaD.: J. Mol. Biol. 161 (1982), 177.
DosterW., and CusackS.: Experimental Report No. 8-04-110, Institut Laue-Langevin, Grenoble, France (1987).
SundaralingamM., DrendelW., and GreaserM.: Proc. Natl. Acad. Sci. USA 82 (1985), 7944.
CrickF. H. C.: Nature 170 (1952), 882.
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Martel, P., Lin, H. Large-scale oscillatory motions in myoglobin. J Biol Phys 17, 137–144 (1989). https://doi.org/10.1007/BF00417754
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DOI: https://doi.org/10.1007/BF00417754