Summary
Biochemical conditions regulating the 11β-hydroxysteroid dehydrogenase activity in spleen homogenate of mice have been studied. The conversion of 11-hydroxy- to 11-dehydrosteroids is a reversible reaction. The ratio of these compounds at equilibrium and the reaction rates depend on the pH and on the proportion between NADP and NADPH (or NAD and NADH) in the incubation medium. Although having identical maximal reaction velocities, the conversion rate below this maximal concentration is much greater for corticosterone than for cortisol or allotetrahydrocortisol.
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The following abbreviations were used: allotetrahydrocortisol (or 5 α-H4F): 3α, 11β, 17α, 21-tetrahydroxy-5α-pregnan-20-one; 11-dehydrocorticosterone (or A): 21-hydroxypregn-4-ene-3,11,20-trione.
Supported in part by grant no 449 of the “National Fonds voor Geneeskundig Wetenschappelijk Onderzoek”.
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Deckx, R., de Moor, P. Study of the 11 β-hydroxysteroid dehydrogenase in vitro. Pflügers Arch. 289, 59–68 (1966). https://doi.org/10.1007/BF00417644
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DOI: https://doi.org/10.1007/BF00417644