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Protein mass transfer studies on a spray column using the PEG-Reppal PES 100 aqueous two-phase system

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Abstract

The characterization of Bovine Serum Albumin mass transfer mechanisms in a spray column using an aqueous two-phase system composed of poly(ethylene glycol) and a modified starch-Reppal PES 100-is done. The poly(ethylene glycol) rich phase is used as the dispersed phase and protein transfer takes place from the dispersed phase to the continuous phase. The effect of dispersed phase superficial velocity, system composition, continuous phase height and distribution system design on either overall protein mass transfer coefficient or column hold-up is described. It is shown that continuous phase superficial velocity and phase composition are the main controlling factors for protein transfer. It is also observed that, with the tested system, only at very low dispersed phase superficial velocities is it possible to operate the spray column as an extraction column. In this system the upper operating limit of the dispersed phase velocity is ten times smaller than in other aqueous two-phase systems.

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Abbreviations

ATPS:

Aqueous Two-Phase System

BSA:

Bovine Serum Albumin

C i kg m−3 :

inlet dispersed phase protein concentration

C 0 kg m−3 :

outlet dispersed phase protein concentration

C d kg m−3 :

dispersed phase protein concentration

C c kg m−3 :

continuous phase protein concentration

D m:

column internal diameter

H :

hold-up

h, h d m:

dispersion height

h 0 m:

initial dispersion height (initial continuous phase height)

k da s−1 :

overall mass transfer coefficient

m :

protein partition coefficient

n :

number of holes of distribution system

PEG:

Poly(ethylene glycol)

Q m3 s−1 :

dispersed phase volumetric flow rate

S m2 :

column internal area

V m3 :

dispersion volume

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Authors and Affiliations

  1. Centro de Engenharia Biológica da Universidade do Minho, Campus de Gualtar, 4709, Braga codex, Portugal

    A. Venâncio & J. A. Teixeira

Authors
  1. A. Venâncio
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  2. J. A. Teixeira
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A. Venâncio was supported by a JNICT (Junta Nacional de Investigaçäo Científica e Tecnológica) grant.

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Venâncio, A., Teixeira, J.A. Protein mass transfer studies on a spray column using the PEG-Reppal PES 100 aqueous two-phase system. Bioprocess Engineering 13, 251–255 (1995). https://doi.org/10.1007/BF00417636

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  • DOI: https://doi.org/10.1007/BF00417636

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