Abstract
A novel RNA fraction, ‘Cy RNA,’ that accumulates in mitochondria when hamster cells are treated with the methylation inhibitor cycloleucine, has been characterized by high resolution acrylamide gel electrophoresis and DNA-RNA hybridization. Cy RNA ran in gels as a discrete band, with an apparent chain length of 2 600. It hybridized specifically to restriction fragments containing genes for the mitochondrial ribosomal RNAs. We infer that Cy RNA is a discrete polycistronic ribosomal RNA precursor transcript, whose processing is dependent on normal methylation.
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Prince, D.L., Kotin, R.M. & Dubin, D.T. Evidence that the methylation inhibitor cycloleucine causes accumulation of a discrete ribosomal RNA precursor in hamster mitochondria. Mol Biol Rep 11, 51–55 (1986). https://doi.org/10.1007/BF00417596
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DOI: https://doi.org/10.1007/BF00417596