Abstract
Treatment of 60S subunits from yeast ribosomes with dicarboxylic acid anhydrides (maleic, dimethylmaleic and tetrahydrophtalic), which introduces negatively-charged residues, is accompanied by substantial dissociation of protein components (35–55%). In contrast, acetic anhydride or cyanate, which introduce uncharged groups, cause practically no protein release, even after extensive modification. Therefore, in addition to blocking lysine-RNA interactions, a large change in the electric charge of the proteins appears to be necessary to obtain dissociation. These results seem to indicate that lysine residues are not essential to ribosome integrity, while arginine-RNA interactions should play an important role in the maintenance of ribosomal structure.
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Vioque, A., Hernández, F. & Palacián, E. Effects of different amino-group reagents on ribosomal integrity: structural role of lysine residues. Mol Biol Rep 11, 47–50 (1986). https://doi.org/10.1007/BF00417595
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DOI: https://doi.org/10.1007/BF00417595