Summary
Minimal erythema dose of ultraviolet light correlated significantly with the UV-sensitivity of fibroblast cells from 5 patients with xeroderma pigmentosum, 13 patients with keratinocytic neoplasms of the skin, and 21 control subjects, but not with that of cells from 6 patients with photosensitive dermatitis. In unscheduled DNA synthesis after UV irradiation, the number of grains per nucleus was much less in keratinocytes than in fibroblasts, but the relative doseresponse relationship was similar. This indicates that keratinocytes can also be used in vitro UV-sensitivity studies.
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Supported in part by a Grant for Cancer Research from the Ministry of Education, Science and Culture of Japan
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Machino, H., Shiraishi, S. & Miki, Y. Comparisons of in vivo and in vitro photosensitivities and DNA repair in fibroblast and keratinocyte cells. Arch Dermatol Res 279, 125–129 (1986). https://doi.org/10.1007/BF00417533
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DOI: https://doi.org/10.1007/BF00417533