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Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

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Abstract

Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

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Abbreviations

A650 :

absorbance at 650 nm

TCA:

trichloroacetic acid

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Author notes

  1. Hirokazu Inoue

    Present address: Department of Tumor Viruses, Research Institute for Microbial Diseases, Osaka University, Yamada-kami, 565, Suita, Osaka, Japan

Authors and Affiliations

  1. Department of biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558, Osaka, Japan

    Hirokazu Inoue & Chikashi Shimoda

Authors
  1. Hirokazu Inoue
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  2. Chikashi Shimoda
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Inoue, H., Shimoda, C. Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe . Arch. Microbiol. 129, 19–22 (1981). https://doi.org/10.1007/BF00417172

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  • DOI: https://doi.org/10.1007/BF00417172

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