Archives of Microbiology

, Volume 129, Issue 1, pp 19–22

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

  • Hirokazu Inoue
  • Chikashi Shimoda

DOI: 10.1007/BF00417172

Cite this article as:
Inoue, H. & Shimoda, C. Arch. Microbiol. (1981) 129: 19. doi:10.1007/BF00417172


Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

Key words

Germination Glycogen Outgrowth Schizosaccharomyces pombe Spore Trehatase Trehalose Yeast 



absorbance at 650 nm


trichloroacetic acid


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Hirokazu Inoue
    • 1
  • Chikashi Shimoda
    • 1
  1. 1.Department of biology, Faculty of ScienceOsaka City UniversityOsakaJapan
  2. 2.Department of Tumor Viruses, Research Institute for Microbial DiseasesOsaka UniversitySuita, OsakaJapan

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