Archives of Microbiology

, Volume 129, Issue 1, pp 19–22 | Cite as

Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe

  • Hirokazu Inoue
  • Chikashi Shimoda
Article

Abstract

Quantitative changes in various carbohydrates of Schizosaccharomyces, pombe spores during germination and outgrowth were studied. Trehalose decreased rapidly, shortly after onset of germination, while glycogen remained constant throughout germination and outgrowth. Alkali-insoluble carbohydrates decreased after the lag period of about 40 min. The content of alkali-soluble carbohydrates was constant during germination, but increased remarkably in parallel with germtube formation. The mechanism of rapid degradation of trehalose during germination was also studied. The activity of trehalase (EC 3.2.1.28) was detected only in the cell wall fraction of isolated spores. Trehalase activity in the cell wall fraction was not enhanced during germination. Trehalose was not found in the isolated spore walls, but in the soluble fraction. These facts strongly suggested that trehalose, and trehalase were spatially separated in dormant spores and that trehalase became accessible to trehalose upon germination.

Key words

Germination Glycogen Outgrowth Schizosaccharomyces pombe Spore Trehatase Trehalose Yeast 

Abbreviations

A650

absorbance at 650 nm

TCA

trichloroacetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Dubois M, Gilles K, Hamilton JK, Rebers PA, Smith F (1951) A colorimetric method for the determination of sugars. Nature 168:167PubMedCrossRefGoogle Scholar
  2. Egel R (1971) Physiological aspects of conjugation in fission yeast. Planta 98:89–96CrossRefGoogle Scholar
  3. Gottlieb D (1976) Carbohydrate metabolism and spore germination. In: Weber DJ, Hess WM (eds) The fungal spore. New York, John Wiley and Sons, pp 141–163Google Scholar
  4. Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC (ed) Handbook of genetics, Vol 1. New York Plenum Press, pp 395–446Google Scholar
  5. Hecker LI, Sussman AS (1975) Localization of trehalase in the ascospores of Neurospora: Relation to ascospore dormancy and germination. J Bactriol 115:592–599Google Scholar
  6. Kane SM, Roth R (1974) Carbohydrate metabolism during ascospore development in yeast. J Bacteriol 118:8–14PubMedGoogle Scholar
  7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  8. Nishi K, Shimoda C, Hayashibe M (1978) Germination and outgrowth of Schizosaccharomyces pombe ascospores isolated by Urografin density gradient centrifugation. Can J Microbiol 24:893–897PubMedGoogle Scholar
  9. Padilla GM, Creanor J, Fraser RSS (1974) Early events in the germination of S. pombe ascospores. In: Padilla GM, Cameron IL, Zimmerman A (eds) Cell cycle control. New York Academic Press, pp 167–180Google Scholar
  10. Papadopoulas NM, Hess WC (1960) Determination of neuraminic (sialic) acid, glucose and fructose in spinal fluid. Arch Biochem Biophys 88:167–171CrossRefGoogle Scholar
  11. Rousseau P, Halvorson HO, Bulla Jr, LA, Julian GS (1972) Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy. J Bacteriol 109:1232–1238PubMedGoogle Scholar
  12. Rousseau P, Halvorson HO (1973a) Physiological changes following the breaking of dormancy of Saccharomyces crevisiae ascospores. Can J Microbiol 19:547–555PubMedCrossRefGoogle Scholar
  13. Rousseau P, Halvorson HO (1973b) Effect of metabolic inhibitors on germination and outgrowth of Saccharomyces cerevisiae ascospores. Can J Microbiol 19:1311–1318PubMedGoogle Scholar
  14. Rousseau P, Halvorson HO (1973c) Macromolecular synthesis during the germination of Saccharomyces cerevisiae spores. J Bacteriol 113: 1289–1295PubMedGoogle Scholar
  15. Savarese JJ (1974) Germination studies on pure yeast ascospores. Can J Microbiol 20:1517–1522PubMedGoogle Scholar
  16. Shimoda C (1980) Differential effect of glucose and fructose on spore germination in the fission yeast, Schizosaccharomyces pombe. Can J Microbiol 26:741–745CrossRefGoogle Scholar
  17. Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23Google Scholar
  18. Souza NO, Panek AD (1968) Location of trehalase and trehalose, in yeast cells. Arch Biochem Biophys 125:22–28PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Hirokazu Inoue
    • 1
  • Chikashi Shimoda
    • 1
  1. 1.Department of biology, Faculty of ScienceOsaka City UniversityOsakaJapan

Personalised recommendations