Abstract
A series of deletions beginning in the leu operon and continuing into the araC gene and also into the ara controlling site region were analyzed in reciprocal merodiploids, e.g., F′A2C c67/B24Δ719, F′B24Δ719/A2C c67, for their effects on catabolite deactivation (CD). The results of these experiments are consistent with placing the catabolite gene activator-cyclic AMP sensitive site in the controlling site region between araB and araO. With a deletion mutant, Δ1109, that places araBAD under leu control when transcription begins atleuP, the araBAD operon is immune to CD even though araCGA, araP and aral are intact and functional. To focus attention on the fine structure and related functions of this region we propose that the three proteins that function therein have separate sites of action: araI (initiator-site for activator), araP (promoter-site for RNA polymerase) and ara(CGA) (catabolite gene activator-site for CGA-cAMP). None of the eighteen initiator constitutive mutants (I c) tested have any significant effect on catabolite derepression or on the maximal level of expression of the operon supporting the view that the araI site may be distinct from araP and ara(CGA). A series of constitutive mutants in the araC gene (C c) also have no pronounced effect on catabolite deactivation.
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It is a pleasure to dedicate this paper to Prof. Roger Stanier on the occasion of his 60th birthday
This work was supported in part by a research grant from the National Science Foundation (GB24093)
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Bass, R., Heffernan, L., Sweadner, K. et al. The site for catabolite deactivation in the L-arabinose BAD operon in Escherichia coli B/r. Arch. Microbiol. 110, 135–143 (1976). https://doi.org/10.1007/BF00416978
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DOI: https://doi.org/10.1007/BF00416978