Abstract
A spontaneous mutant of Pseudomonas putida (PRS 2017) has been isolated which is incapable of growth on benzoate, does not induce the enzymes of the catechol branch of the β-ketoadipate pathway when grown in the presence of benzoate, cannot accumulate radioactively labeled benzoate, yet grows well with mandelate as sole source of carbon and energy. This strain apparently lacks a benzoate permease, which in the wild type shows a K mof about 0.1 mM for benzoate, is inducible, and is not under the control of the regulatory system which governs the induction of the enzymes of the catechol branch of the β-ketoadapate pathway. The lesion in PRS 2017 is apparently single site and maps near other genes governing benzoate dissimilation.
Similar content being viewed by others
References
Adams, M. H.: Bacteriophages. New York: Wiley 1966
Hegeman, G. D.: Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes of the wild type. J. Bact. 91, 1140–1154 (1966a)
Hegeman, G. D.: Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. II. Isolation and properties of blocked mutants. J. Bact. 91, 1155–1160 (1966b)
Leidigh, B. J., Wheelis, M. L.: The clustering on the Pseudomonas putida chromosome of genes specifying dissimilatory functions. J. molec. Evol. 2, 235–242 (1973)
Lennox, E. S.: Transduction of linked genetic characters of the host by phage P1. Virology 1, 190–206 (1955)
Meagher, R. B., McCorkle, G. M., Ornston, M. K., Ornston, L. N.: Inducible uptake system for β-carboxy-cis,cis-muconate in a permeability mutant of Pseudomonas putida. J. Bact. 111, 465–473 (1972)
Ornston, L. N.: The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. III. Enzymes of the catechol pathway. J. biol. Chem. 241, 3795–3799 (1966a)
Ornston, L. N.: The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. IV. Regulation. J. biol. Chem. 241, 3800–3810 (1966b)
Reiner, A. M., Hegeman, G. D.: Metabolism of benzoic acid by bacteria. Accumulation of (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid by a mutant strain of Alcaligenes eutrophus. Biochemistry 10, 2530–2536 (1971)
Simon, R. D.: The use of an ultrasonic bath to disrupt cells suspended in volumes less than 100 μl. Analyt. Biochem. 60, 51–58 (1974)
Stanier, R. Y., Palleroni, N. J., Doudoroff, M.: The aerobic pseudomonas; a taxonomic study. J. gen. Microbiol. 43, 159–271 (1966)
de Torrontegui, G., Díaz, R., Wheelis, M. L., Cánovas, J. L.: Supraoperonic clustering of genes specifying glucose dissimilation in Pseudomonas putida. Molec. gen. Genetics 144, 307–311 (1976)
Warburg, O., Christiansen, W.: Isolierung und Kristallisation des Gärungsferments Enolase. Biochem. Z. 131, 384–421 (1942)
Wheelis, M. L.: The genetics of dissimilatory pathways in Pseudomonas. Ann. Rev. Microbiol. 29, 505–524 (1975)
Wheelis, M. L., Ornston, L. N.: Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida: Deletion mapping of cat mutation. J. Bact. 109, 790–795 (1972)
Wheelis, M. L., Stanier, R. Y.: The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics 66, 245–266 (1970)
Wu, C. H., Ornston, M. K., Ornston, L. N.: Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida: Two-point crosses with a regulatory mutant strain. J. Bact. 109, 796–802 (1972)
Wu, T. T.: A model for three point analysis of random general transductions. Genetics 54, 405–410 (1966)
Author information
Authors and Affiliations
Additional information
Dedicated to R. Y. Stanier on the occasion of his 60th birthday
Rights and permissions
About this article
Cite this article
Thayer, J.R., Wheelis, M.L. Characterization of a benzoate permease mutant of Pseudomonas putida . Arch. Microbiol. 110, 37–42 (1976). https://doi.org/10.1007/BF00416966
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00416966