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Arsenic elevates the sister chromatid exchange (SCE) rate in human lymphocytes in vitro

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Summary

The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. The addition of inorganic trivalent arsenic (Na2HAsO4) 10-6M and 2x10-6 M induces a significant and dose-dependent increase of SCE. This phenomenon is as sensitive as the evidence of arsenic-induced chromosomal aberrations in vitro.

Zusammenfassung

Die Zahl von »sister chromatid exchanges« (SCE) pro Metaphase wurde mit der Hoechst-Giemsa Technik an menschlichen Lymphocyten bestimmt. Die Zugabe von anorganischem trivalenten Arsen (Na2HAsO4) 10-6 M und 2x10-6 M zeigt einen signifikanten und dosisabhängigen Anstieg der SCE. Dieses Phänomen ist ebenso empfindlich wie der Nachweis von Chromosomenaberrationen der Lymphocyten durch Arsenexposition in vitro.

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  1. Hautklinik der Städt. Krankenanstalten, Postfach 23, D-6800, Mannheim, Germany

    Flavia Zanzoni & E. G. Jung

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  1. Flavia Zanzoni
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  2. E. G. Jung
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Zanzoni, F., Jung, E.G. Arsenic elevates the sister chromatid exchange (SCE) rate in human lymphocytes in vitro. Arch Dermatol Res 267, 91–95 (1980). https://doi.org/10.1007/BF00416927

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  • DOI: https://doi.org/10.1007/BF00416927

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