Summary
Sera from animals immunized to basement membrane proteins were collected and studied by indirect immunofluorescence using rat skin, esophagus, kidney, urinary bladder, rabbit skin, and human skin as substrates. Serum of immunized rabbits showed indirect immunofluorescent staining at the basement membrane zone of rat skin, esophagus, glomerulus, Bowman's capsule, rabbit skin, and human skin. In addition, the connective tissue sheaths of muscle fibers showed fluorescent staining. Direct immunofluorescence of skin, esophagus, kidney, and urinary bladder obtained from immunized animals was negative in all cases. This study demonstrates the antibody-inducing capability of basement membrane zone antigens of stratified squamous epithelium and suggests the presence of an antigen common to basement membranes of stratified squamous epithelium, glomerulus, Bowman's capsule, and the connective tissue sheaths of striated muscle. Histopathologic abnormalities were not associated with these antibodies in the immunized animals.
Zusammenfassung
Es wurden die Seren von Tieren, die gegen Basalmembran-Proteine immunisiert waren, gesammelt und in der indirekten Immunfluorescenz untersucht. Als Substrat wurde die Haut, Oesophagus, Nieren und Harnblasen von Ratten, sowie Kaninchen- und Menschenhaut benutzt. Mit dem Serum immunisierter Kaninchen zeigten sich Immunglobulin-Ablagerungen an der Basalmembran der Haut, Oesophagus, Glomerula und Bowmanschen Kapseln von Ratten, ebenfalls an der Kaninchen- und Menschenhaut. Außerdem zeigten die Muskelfascien eine Fluorescenz. Die direkte Immunfluorescenz der Haut, Oesophagus, Nieren und Harnblasen der immunisierten Tiere war in allen Fällen negativ. Diese Studie zeigt die Antikörper induzierende Fähigkeit der Antigene der Basalmembran-Zone des Plattenepithels und legt den Gedanken nahe, daß ein Antigen vorliegen muß, das gleichermaßen gegen Basalmembranen des Plattenepithels, Glomerula und Bowmansche Kapsel sowie gegen die bindegewebigen Muskelfascien der quergestreiften Muskeln gerichtet ist. Histopathologische Abnormalitäten waren nicht mit den Antikörpern der immunisierten Tiere vergesellschaftet.
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Cannon, J., Persellin, R. Experimental production of basement membrane zone antibodies. Arch Dermatol Res 267, 71–78 (1980). https://doi.org/10.1007/BF00416923
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DOI: https://doi.org/10.1007/BF00416923