Summary
A reversible dissociation of the protein-lipopolysaccharide complex (Pr-LPS) isolated from Pseudomonas aeruginosa after sodium deoxycholate treatment was demonstrated by ultracentrifugation, gel filtration and immunological techniques. Removal of deoxycholate from the antigen solution restored it to a molecular weight similar to the original substance. In addition, subunits of the antigen seem to retain the ability to precipitate with anti Pr-LPS serum.
Neither high concentrations of polymyxin B nor surfactants as Triton X-100 or Tween-80 produced this molecular dissociation in the complex.
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Rubio, N., Portolés, A. & Lopez, R. Immuno-physical properties of Pseudomonas aeruginosa protein-lipopolysaccharide complex treated with surfactants. Archiv. Mikrobiol. 94, 149–158 (1973). https://doi.org/10.1007/BF00416689
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DOI: https://doi.org/10.1007/BF00416689