Summary
A diffusible self-inhibitor of germination of conidia of Glomerella cingulata appears to act as a regulator of protein synthesis. Both uptake of labeled amino acids and their incorporation into protein are reduced by the inhibitor or by crowding. Compared to conidia incubated without self-inhibitor, conidia incubated with self-inhibitor incorporated no labeled amino acids into protein in the first hour and 80% less in 6h. Thoroughly washed conidia were more permeable to amino acids and incorporated 6 times more precursor into proteins than unwashed conidia. At high density in nutrient medium, conidia of G. cingulata preferentially form secondary conidia instead of germ tubes and a mycelium. This inhibition of germination of conidia and regulation of development is mimicked by exposing them to an auto-inhibitor extracted from used culture medium and conidial washings. Germination of conidia of G. cingulata involves two steps, an initial step of 5 h duration which continues unaffected by crowing (1.7×108/ml) and a subsequent 2 h step which conidia do not take unless they are sufficiently diluted. It is this step for which protein synthesis may be required.
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Abbreviations
- CHM:
-
cyloheximide
- NM:
-
Neurospora minimal medium
- psi:
-
pound per square inch
- RPH:
-
reconstituted algal protein hydrolysate
- TCA:
-
trichloroacetic acid
References
Allen, P. J.: Metabolic aspects of spore germination in fungi. Ann. Rev. Phytopath. 3, 313–342 (1965)
Cochrane, V. W.: Physiology of fungi. New York: Wiley 1958
Johnson, M. J.: Isolation and properties of a pure yeast polypeptidase. J. biol. Chem. 137, 575–586 (1941)
Lingappa, B. T., Lingappa, Y.: Effects of nutrients on self-inhibition of germination of conidia of Glomerella cingulata. J. gen. Microbiol. 41, 67–75 (1965)
Lingappa, B. T., Lingappa, Y.: The nature of self-inhibition of germination of conidia of Glomerella cingulata. J. gen. Microbiol. 43, 91–100 (1966)
Lingappa, B. T., Lingappa, Y.: Role of auto-inhibitors on mycelial growth and dimorphism of Glomerella cingulata. J. gen. Microbiol. 56, 35–45 (1969)
Lingappa, B. T., Prasad, M., Lingappa, Y., Hunt, D. F., Biemann, K.: Phenethyl alcohol and tryptophol: Autoantibiotics produced by the fungus Candida albicans. Science 163, 192–194 (1969)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mackintosh, F. R., Bell, E.: Regulation of protein synthesis in sea urchin eggs. J. molec. Biol. 41, 365–380 (1969)
Macko, V., Staples, R. C., Allen, P. J., Renwick, J. A. A.: Identification of the germination self-inhibitor from wheat stem rust uredospore. Science 173, 835–836 (1971)
Macko, V., Staples, R. C., Gershon, H., Renwick, J. A. A.: Self-inhibitor of bean rust uredospores: Methyl 3,4-dimethoxycinnamate. Science 170, 539–540 (1970)
Mandels, G. R.: Biotin and interrupted growth of Myrothecium verrucaria. Amer. J. Bot. 42, 921–929 (1955)
Padwick, G. W.: Note on the limitation of infection of wheat by ascospores of Ophiobolus graminis Saac. A possible explantation. Ann. Biol. 26, 823–825 (1939)
Richardson, L. T., Thorn, G. D.: Stimulation of spore germination and growth of Glomerella cingulata by copper and other heavy metal ions. Phytopathology 52, 865–869 (1962)
Schmoyer, I. R., Lovett, J. S.: Regulation of protein synthesis in zoospores of Blastocladiella. J. Bact. 100, 854–864 (1969)
Smart, R. F.: Influence of certain external factors on spore germination in the myxomycetes. Amer. J. Bot. 24, 145–159 (1937)
Stevens, F. L., Hall, J. G.: Variation of fungi due to environment. Bot. Gaz. 48, 1–30 (1909)
Van Ettan, J. L.: Protein synthesis during fungal spore germination. Phytopathology 59, 1060–1064 (1969)
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Lingappa, B.T., Lingappa, Y. & Bell, E. A self-inhibitor of protein synthesis in the conidia of Glomerella cingulata . Archiv. Mikrobiol. 94, 97–107 (1973). https://doi.org/10.1007/BF00416685
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DOI: https://doi.org/10.1007/BF00416685