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Differential expression of “Fe-repressible” and “growth-rate-sensitive” proteins in Neisseria meningitidis and Neisseria gonorrhoeae

  • Section 5: Pathogenesis
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Abstract

By varying growth conditions, we were able to differentiate (SDS-PAGE) between true “Fe-repressible” proteins and “growth-rate-sensitive” proteins (GSP's) (60-110 Kd) in outer membranes of iron-starved meningococci and gonococci. Immunological characterization with monoclonal antibodies raised against the GSP's revealed (GIRA) crossreactivity between different GSP's. On the basis of common epitopes, two groups of proteins could be distinguished. Immuno-electron-microscopy showed that proteins of both groups were exposed at the cell surface.

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Authors and Affiliations

  1. Laboratory of Medical Microbiology, Academic Medical Center, University of Amsterdam, The Netherlands

    J. P. M. Van Putten, M. T. J. Linders & J. F. L. Weel

  2. The National Institute of Public Health and Environmental Protection (RIVM), Bilthoven, The Netherlands

    J. T. Poolman

Authors
  1. J. P. M. Van Putten
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  2. M. T. J. Linders
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  3. J. F. L. Weel
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  4. J. T. Poolman
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Van Putten, J.P.M., Linders, M.T.J., Weel, J.F.L. et al. Differential expression of “Fe-repressible” and “growth-rate-sensitive” proteins in Neisseria meningitidis and Neisseria gonorrhoeae . Antonie van Leeuwenhoek 53, 557–564 (1987). https://doi.org/10.1007/BF00415517

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