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Characterization of the major iron-regulated protein of Neisseria gonorrhoeae and Neissereria meningitidis

  • Section 3: Outer Membrane Proteins and IgA Protease
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Abstract

The major iron-regulated protein (MIRP) was purified, from both Neisseria gonorrhoeae and N. meningitidis by selective extraction with cetyltrimethylammonium bromide followed by ion-exchange and moleculair-seive chromatography. Solutions of the purified proteins had a characteristic pink color. The overall amino acid composition of these proteins was similar, although differences were noted in the number of serine, threonine, and lysine residues. Nevertheless, the N-terminal amino acid sequence was identical through 47 residues for both the meningococcal and gonococcal MIRP. Plasma emission spectrophotometry revealed that the meningococcal 37K protein contained ca. 1 mole Fe/mole protein.

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References

  • Blake, M. S. & E. C. Gotschlich (1982) Purification and partial characterization of the major outer membrane protein of Neisseria gonorrhoeae. Infect. Immunity 36: 277–283

    Google Scholar 

  • Blake, M. S. & E. C. Gotschlich (1984) Purification and characterization of the opacity-associated proteins of Neisseria gonorrhoeae. J. Exp. Med. 154: 452–462

    Google Scholar 

  • Campbell, H. D. & B. Zerner (1973) A low-molecular-weight acid phosphatase which contains iron. Biochem. Biophys. Res. Commun. 54: 1498–1503

    Google Scholar 

  • Mietzner, T. A., R. C. Barnes, Y. A. JeanLouis, W. M. Shafer & S. A. Morsé (1986) Distribution of an antigenically related iron-regulated protein among the Neisseria spp. Infect. Immunity 51: 60–68

    Google Scholar 

  • Mietzner, T. A., G. H. Luginbuhl, E. Sandstrom & S. A. Morse (1984) Identification of an iron-regulated 37,000-dalton protein in the cell envelope of Neisseria gonorrhoeae. Infect. Immunity 45: 410–416

    Google Scholar 

  • Mietzner, T. A. & S. A. Morse (1985) Iron-regulated membrane proteins of Neisseria gonorrhoeae: purification and partial characterization of a 37,000-dalton protein. In: G. Schoolnik, G. F. Brooks, S. Falkow, C. E. Frasch, J. S. Knapp, J.A. McCutchen & S.A. Morse (Ed) The Pathogenic Neisseriae (pp. 406–414). American Society for Microbiology, Washington, D.C.

    Google Scholar 

  • West, S. E. H. & P. F. Sparling (1985) Response of Neisseria gonorhoeae to iron limitation: alterations in expression of membrane proteins without apparent siderophore production. Infect. Immunity 47: 388–394

    Google Scholar 

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Authors and Affiliations

  1. Center for Infectious Diseases, Sexually Transmitted Diseases Laboratory Program, Centers for Disease Control, 30333, Atlanta, GA, USA

    S. A. Morse & A. Le Faou

  2. Dept. of Medical Microbiology, Stanford University, 94305, Stanford, CA, USA

    T. A. Mietzner, G. Bolen & G. Schoolnik

Authors
  1. S. A. Morse
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  2. T. A. Mietzner
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  3. G. Bolen
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  4. A. Le Faou
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  5. G. Schoolnik
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Morse, S.A., Mietzner, T.A., Bolen, G. et al. Characterization of the major iron-regulated protein of Neisseria gonorrhoeae and Neissereria meningitidis . Antonie van Leeuwenhoek 53, 465–469 (1987). https://doi.org/10.1007/BF00415504

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  • DOI: https://doi.org/10.1007/BF00415504

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