Abstract
There has been a decrease in the prevalence of disease in the United States due to meningococcal serotypes 2a and 2b containing class 2 proteins with a concomitant increase in nonserotypable strains containing class 3 major outer membrane proteins. A new disease associated strain was identified using monoclonal antibodies as B:4:P1.15. Serotype 4 strains have been heretofore solated almost only from carriers. This B:4:P1.15 strain predominated among group B disease isolates in Cuba from the late 1970s to the present and among Miami, Florida isolates recovered in 1981 and 1982. To determine whether protein vaccines for new strains or serotypes could be prepared using our present methods, a combined vaccine was prepared from a group B strain (B:8:P1.15) recovered during a recent outbreak in Virginia, and a serotype 2b strain, plus group C polysaccharide. The vaccine was prepared with aluminum hydroxide, or with trehalose dimycolate plus monophosphoryl lipid A, or without adjuvant. Four weeks after immunization antibody levels were much higher in mice that received vaccine containing adjuvant.
Article PDF
Similar content being viewed by others
References
Caugant, D. A., L. O. Froholm, K. Bovre, E. Holten, C. E. Frasch, L. F. Mocca, W. D. Zollinger & R. K. Selander (1986) Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease. Proc. Natl. Acad. Sci. USA 83: 4927–4931
Caugant, D. A., F. L. Mocca, C. E. Frasch, L. O. Froholm, W. D. Zollinger & R. K. Selander (1987) Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. J. Bacteriol 169: 2781–2792
Craven, D. E., C. E. Frasch, L. F. Mocca, F. B. Rose & R. Gonzalez (1979) Rapid serogroup identification of Neisseria meningiditis by using antiserum agar: prevalence of serotypes in a disease-free military population. J. Clin. Microbiol. 10: 302–307
Frasch, C. E. & S. S. Chapman (1973) Classification of Neisseria meningitidis group b into distinct serotypes. III. Application of a new bactericidal inhibition technique to distribution of serotypes among cases and carriers. J. Infect. Dis. 127: 149–154
Frasch, C. E., G. Coetzee, J. M. Zahradnik & L. Y. Wang (1985a) New developments in meningococcal vaccines. In: G. K. Schoolnik (Ed) The pathogenic Neisseria (pp. 633–639). ASM, Washington, D. C.
Frasch, C. E., W. D. Zollinger & J. T. Poolman (1985b) Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. Infec. Dis. 7: 504–510.
Frasch, C. E. & J. M. Zahradnik (1986) Human immune response to an aluminum hydroxide absorbed Neisseria meningiditis serotype 2b protein vaccine. Antonie van Leeuwenhoek 52: 229–231
Froholm, L. O., E. Holten, J. T. Poolman & W. D. Zollinger (1985) Typing of Norwegian meningococcal isolates and possible implications for serogroup B vaccination. In: G. K. Schoolnik (Ed) The Pathogenic Neisseria (pp. 541–545). ASM, Washington, D. C.
Hawkes, R. E. Niday & J. Gordon (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 119: 142–147
Mocca, L. F. & C. E. Frasch (1982) Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. J. Clin. Microbiol. 16: 240–244.
Peppler, M. S. & C. E. Frasch (1982) Proetection against group B Neisseria meningitidis disease: Effect of serogroup B polysaccharide and polymyxin B on immunogenicity of serotype protein preparations. Infect. Immun. 37: 264–270
Tsai, C-M, C. E. Frasch & L. F. Mocca (1981) Five structural classes of major outer membrane proteins in Neisseria meningitidis. J. Bacteriol. 146: 69–78
Wang, L Y. & C. E. Frasch (1984) Development of a Neisseria meningitidis group B serotype 2b protein vaccine and evaluation in a mouse model. Infect. Immun. 46: 408–414
Zollinger, W. D. & R. E. Mandrell (1983) Studies of the human antibody response to specific meningococcal outer membrane proteins of serotypes 2 and 15. Med. Tropicale 43: 143–147
Zollinger, W. D., R. E. Mandrell, J. M. Griffiss, P. Alteri & S. Berman (1979) Complex of meningococcal group B polysaccharide and type 2 outer membrane protein immunogenic in man. J. Clin. Invest. 63: 836–848
Zollinger, W. D., E. E. Moran, H. Connely, R. E. Mandrell & B. Brandt (1984) Monoclonal antibodies to serotype 2 and 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping. Infect. Immun. 46: 260–266
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Frasch, C.E., Mocca, L.F. & Karpas, A.B. Appearance of new strains associated with group B meningococcal disease and their use for rapid vaccine development. Antonie van Leeuwenhoek 53, 395–402 (1987). https://doi.org/10.1007/BF00415493
Issue Date:
DOI: https://doi.org/10.1007/BF00415493