Summary
Great Chinchilla rabbits (body weight 1.5–2.5 kg) were given interscapularly a single immunizing dose of, respectively: a) 50 mg (wet weight) of freshly dissected homologous retina emulsified in 0.4 ml of Freund's complete adjuvant (110 animals); b) 50 mg homologous retina as above incorporated in 0.4 ml saline (10 animals); c) 50 mg homologous retina as above incorporated in 0.4 ml of Freund's incomplete adjuvant (10 animals); d) 0.4 ml Freund's adjuvant (20 animals).
Before starting the experiment, and at daily intervals thereafter, all animals were examined for eye lesions (ophthalmoscope, slit lamp) and for neurological deficits. In 90 experimental and in all control animals these examinations were carried out over a period of 40 days.
63 of 93 experimental animals developed ocular lesions characterized by chorioretinic patches of varying size in all parts of the fundus. The time of disease onset varied from 12 to 39 days after immunization. The most violent inflammations appeared as a rule in the third week after inoculation and reached maximum severity within 60 hours. Three types of clinical pictures are described. No involvement of the anterior segment or of the central nervous system was noted clinically in any of the animals. In contrast to the control preparations tested (b, c, d), only homologous retina in Freund's complete adjuvant was effective.
Zusammenfassung
Groß-Chinchilla-Kaninchen (1,5–2,5 kg KG) erhielten interscapulär eine einzige Injektion von respektive:
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a)
Einer Emulsion von 50 mg (Naßgewicht) frischer homologer Retina in 0,4 ml kompletten Freundschen Adjuvans (110 Tiere);
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b)
einer Suspension von 50 mg homogenisierter, frischer homologer Retina in 0,4 ml physiologischer Kochsalzlösung (10 Tiere);
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c)
einer Emulsion von 50 mg frischer homologer Retina in 0,4 ml inkompletten Freundschen Adjuvans (10 Tiere);
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d)
0,4 ml kompletten Freundschen Adjuvans ohne Retina (20 Tiere).
Vor Beginn des Versuchs wurden alle Kaninchen und post injectionem 90 Versuchs-sowie sämtliche Kontrolltiere über 40 Tage täglich auf Augenveränderungen (Ophthalmoskop, Spaltleuchte) und neurologische Ausfälle untersucht. 63 von 90 über die volle Dauer des Experiments beobachteten Tiere reagierten nach einer Latenzzeit von 12–39 Tagen mit einer Chorioretinitis. Die schwersten Entzündungen traten in der Regel während der 3. Woche post injectionem auf und erreichten binnen 60 Std ihr Maximum. Die klinischen Bilder der Erkrankung werden beschrieben und in drei Erscheinungsformen gruppiert. Klinische Zeichen einer Beteiligung der vorderen Augenabschnitte oder des ZNS wurden nicht gefunden. Die beschriebenen Veränderungen waren ausschließlich nach Injektion homologer Retina in komplettem Freundschen Adjuvans, jedoch nicht nach Injektion der Kontrollpräparate b, c, und d zu beobachten.
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Schröder, K.D., Meyer, W., Hempel, E. et al. Experimentell allergische Chorioretinitis bei Kaninchen durch Immunisierung mit homologer Retina in Adjuvans. Albrecht von Graefes Arch. Klin. Ophthalmol. 191, 239–246 (1974). https://doi.org/10.1007/BF00414949
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DOI: https://doi.org/10.1007/BF00414949