Abstract
A simple procedure to obtain plasmid preparations, suitable for transfecting mammalian cell lines using a calcium phosphate co-precipitation technique, is described. The protocol is based on the purification of plasmid DNA by double gel-filtration chromatography on Sephacryl S-1000 and additional slight modifications to the original transfection procedure. The purity of plasmid preparation was verified by analytical methods. The resulting preparation efficiently transfected NIH-3T3 cells.
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Additional information
The authors are with the National Center for Scientific Research, Molecular Biology Department, Biotechnology Branch, POB 6880, Havana, Cuba.
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López-Cánovas, L., Rodríguez, M. & Riverón Rojas, A.M. A simple procedure to obtain plasmids suitable for transfecting mammalian cell lines. World Journal of Microbiology & Biotechnology 10, 346–347 (1994). https://doi.org/10.1007/BF00414877
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DOI: https://doi.org/10.1007/BF00414877