Abstract
An endopolygalacturonase of Rhizopus sp. strain LKN, one of several isolates from tempe starter (ragi), was purified 235-fold by CM-Sephadex C-50, DEAE-Sephadex A-50 ion exchange chromatographies and Sephadex G-75 gel filtration. The purified enzyme was homogeneous by SDS-PAGE with a M r of 38.5 kDa. Its K m value for pectic acid was 2 mg/ml. It was stable at pH 4.5 to 11 and up to 50°C, with optimum activity at pH 4.5 to 4.75 and 55 to 60°C. Some ionic compounds enhanced the enzyme activity, whereas tannic acid at 0.5 mm caused about 90% inhibition.
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References
Archer, S.A. & Fielding, A.H. 1979 Polygalacturonase isoenzymes of fungi involved in the breakdown of sulphited strawberries. Journal of the Science of Food and Agriculture 30, 711–723.
Chesson, A. 1980 Maceration in relation to the post-harvest handling and processing of plant material. Journal of Applied Bacteriology 48, 1–45
hartree, E.F. 1972 Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48, 422–427
Lee, S.C. & West, C.A. 1981 Properties of Rhizopus stolonifer polygalacturonase, an elicitor of casbene synthetase activity in castor bean (Ricinus communis L.) seedlings. Plant Physiology 67 640–645.
Liu, Y.K. & Luh, B.S. 1978 Purification and characterization of endo-polygalacturonase from Rhizopus arrhizus. Journal of Food Science 43, 721–726.
Luh, B.S., Ozbilgin, S. & Liu, Y.K. 1978 Textural changes in canned apricots in the presence of mold polygalacturonase. Journal of Food Science 43, 713–720.
Manachini, P.L., Fortuna, M.G. & Parini, C. 1987 Purification and properties of an endopolygalacturonase produced by Rhizopus stolonifer. Biotechnology Letters 9, 219–224.
Miller, G.L. 1959 Dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426–428.
Nout, M.J.R. & Rombouts, F.M. 1990 Recent developments in tempe research. Journal of Applied Bacteriology 69, 609–633.
Steinkraus, K.H., Cullen, R.E., Pederson, C.S., Nellis, L.F. & Gavitt, B.K. 1983 Indonesian tempe and related fermentation. In Handbook of Indigenous Fermented Foods, ed Steinkraus, K.H. et al. pp. 1–94. New York: Marcel Dekker.
Trescott, A.S. & Tampion, J. 1974 Properties of endopolygalacturonase secreted by Rhizopus stolonifer. Journal of General Microbiology 80, 401–409.
Weber, K. & Osborn, M. 1969 The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. The Journal of Biological Chemistry 244, 4406–4412.
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The authors are with the Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812, Japan.
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Elegado, F.B., Fujio, Y. Purification and some properties of endopolygalacturonase from Rhizopus sp. LKN. World Journal of Microbiology & Biotechnology 10, 256–259 (1994). https://doi.org/10.1007/BF00414857
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DOI: https://doi.org/10.1007/BF00414857