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Quantitative Bestimmung von Kammerwasserproteinen durch die Elektroimmundiffusion in einem antiserumhaltigen Agarosegel

Quantitative determination of aqueous humor proteins by electroimmodiffusion in an antiserum containing agarosegel

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Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Summary

An exact quantitation of aqueous humor proteins within a few hours can be attained by an modification of the quantitative estimation of the proteins by electrophoresis in agarose gel established by Laurell.

The aqueous humor proteins form long immunoprecipitations in agargel.

The length of the precipitats is proportional to the concentration.

By determination of albumin, coeruloplasmin and transferrin the application can be pointed out.

Zusammenfassung

Eine exakte, quantitative Bestimmung von Kammerwasserproteinen innerhalb weniger Stunden wird durch eine Modifikation der Elektroimmundiffusion nach Laurell erreicht. Dabei hinterläßt das zu untersuchende Kammerwasserprotein bei seiner elektrophoretischen Wanderung im antiserumhaltigen Agarosegel langgestreckte Immunpräzipitate, deren Länge proportional zur Konzentration ist.

Anhand der Bestimmung von Albumin, Coeruloplasmin und Transferrin werden die Anwendungsmöglichkeiten aufgezeigt.

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Literatur

  1. Esser, H., Heinzler, F., Pau, H.: Elektrophoretische Trennung der Eiweißfraktionen im menschlichen Kammerwasser. Albrecht v. Graefes Arch. Ophthal. 155, 11–16 (1954)

    Google Scholar 

  2. Francois, J., Rabaey, M., Evens, L.: Agar microelectrophoresis of the aqueous humor. Arch. Ophthal. 59, 692–702 (1958)

    Google Scholar 

  3. Peretz, W. L., Tomasi, T. B.: Aqueous humor proteins in uveitis. Arch. Ophthal. 65 20–23 (1961)

    Google Scholar 

  4. Lowry, O. H., Bosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  5. Meulemans, O.: Determination of total protein in spinal fluid with sulphosalicylic acid and trichloracetic acid. Clin. chim. Acta 5, 757–761 (1960)

    Google Scholar 

  6. Zirm, M., Schmut, O.: Konzentrationsabhängigkeit der Immunglobuline des menschlichen Kammerwassers von Alter und Geschlecht. Albrecht v. Graefes Arch. klin. exp. Ophthal. 188, 239–243 (1973)

    Google Scholar 

  7. Mancini, G., Carbonara, A. O., Heremans, J. F.: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–254 (1965)

    Google Scholar 

  8. Laurell, C. B.: Quantitative estimation of proteins by electrophoresis in agarosegel containing antibodies. Analyt. Biochem. 15, 45–52 (1966)

    Google Scholar 

  9. Weeke, B.: Carbamylated human immunoglobulins tested by electrophoresis in agarose and antibody-containing agarose. Scand. J. clin. Lab. Invest. 21, 351–354 (1968)

    Google Scholar 

  10. Weeke, B.: Quantitative estimation of human immunoglobulins following carbamylation by electrophoresis in antibody-containing agarose. Scand. J. clin. Lab. Invest. 22, 107–111 (1968)

    Google Scholar 

  11. Christiansen, A. H.: A new method for determination of insulin-binding immunoglobulins in insulin-treated diabetic patients. Horm. Metab. Ees. 2, 187–188 (1970)

    Google Scholar 

  12. Christiansen, A. H.: Determination of insulin binding to β-lipoprotein in sera from normals and diabetics applying immunoelectrophoresis. Protides of the Biological Fluids 19, 557–564 (1971)

    Google Scholar 

  13. Nörgaard-Pedersen, B.: Purification and sensitive immunoelectrophoretical detection and quantitation of human α1-foetoprotein. Clin. chim. Acta 88, 163–170 (1972)

    Google Scholar 

  14. Weeke, B.: Quantitative Immunelektrophorese I. „Eaketenimmunelektrophorese.“ Ärztl. Lab. 18, 12–19 (1972)

    Google Scholar 

  15. Schmut, O., Zirm, M.: Vergleichende Untersuchungen über die Normalverteilung der Immunglobuline im menschlichen Serum und Kammerwasser. Klin. Mbl. Augenheilk. 164, 368–371 (1974)

    Google Scholar 

  16. Schmut, O., Zirm, M.: Quantitative Bestimmung verschiedener Kammerwasserproteine. Albrecht v. Graefes Arch. klin. exp. Ophthal. 190, 63–67 (1974)

    Google Scholar 

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Authors and Affiliations

  1. Universitäts-Augenklinik Graz, Österreich

    M. Zirm, W. Petek, O. Schmut, H. Hofmann & A. Holasek

  2. Institut für Medizinische Biochemie, Deutschland

    M. Zirm, W. Petek, O. Schmut, H. Hofmann & A. Holasek

Authors
  1. M. Zirm
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  2. W. Petek
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  3. O. Schmut
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  4. H. Hofmann
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  5. A. Holasek
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Zirm, M., Petek, W., Schmut, O. et al. Quantitative Bestimmung von Kammerwasserproteinen durch die Elektroimmundiffusion in einem antiserumhaltigen Agarosegel. Albrecht von Graefes Arch. Klin. Ophthalmol. 196, 127–132 (1975). https://doi.org/10.1007/BF00414798

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  • DOI: https://doi.org/10.1007/BF00414798

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