Skip to main content
Log in

The internal pH of Acetobacterium wieringae and Acetobacter aceti during growth and production of acetic acid

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

The intracellular pH was measured in growing Acetobacterium wieringae and Acetobacter aceti with an acid equilibrium distribution method. [14C]-acetylsalicylic acid, [14C-benzoic acid and [14C]-acetic acid were used as ΔpH-indicators. The extracellular pH of Acetobacterium wieringae decreased from 7.0 to 5.0 during growth; accordingly, the intracellular pH changed from 7.1 to 5.5, and a ΔpH between 0.1 and 0.65 (interior more alkaline) was maintained. Corresponding results were obtained for Acetobacter aceti. The external pH and the internal pH decreased in parallel from 6.2 to 3.5 and from 5.8 to 3.9, respectively.

This demonstrates that neither the anaerobic nor the aerobic acetogen was able to maintain a large ΔpH in the presence of high concentrations of acetic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adachi O, Shinagawa E, Matsushita K, Ameyama M (1982) Crystallization of membrane-bound alcohol dehydrogenase of acetic acid bacteria. Agric Biol Chem 46:2859–2863

    Google Scholar 

  • Ameyama M, Osada K, Shinagawa E, Matsushita K, Adachi O (1981) Purification and characterization of aldehyde dehydrogenase of Acetobacter aceti. Agric Biol Chem 45:1889–1890

    Google Scholar 

  • Bakker EP, Mangerich W (1981) Interconversion of components of the bacterial proton motive force by electrogenic potassium transport. J Bacteriol 147:820–826

    Google Scholar 

  • Bakker EP, Rottenberg, H, Caplan R (1976) An estimation of the light-induced electrochemical potential difference of protons across the membrane of Halobacterium halobium. Biochim Biophys Acta 440:557–572

    Google Scholar 

  • Baronofsky JJ, Schreurs WJA, Kashket ER (1984) Uncoupling by acetic acid limits growth of and acetogenesis by Clostridium thermoaceticum. Appl Environ Microbiol 48:1134–1139

    Google Scholar 

  • Braun M, Gottschalk G (1982) Acetobacterium wieringae sp.nov., a new species producing acetic acid from molecular hydrogen and carbon dioxide. Zbl Bakt Hyg I. Abt Orig C 3:368–376

    Google Scholar 

  • Brink B, Konings WN (1982) Electrochemical proton gradient and lactate concentration in Streptococcus cremoris cells grown in batch culture. J Bacteriol 152:682–686

    Google Scholar 

  • Bryant MP (1972) Commentary on the hungate-technique for culture of anaerobic bacteria. Am J Clin Nutr 25:1324–1328

    Google Scholar 

  • Caldwell PC (1956) Intracellular pH. Int Rev Cytol 5:229

    Google Scholar 

  • Foda O, Vaughn RH (1953) The nutritional requirements of Acetobacter melanogenum and related species. J Bacteriol 65: 78–82

    Google Scholar 

  • Gottwald M, Gottschalk G (1985) The internal pH of Clostridium acetobutylicum and its effect on the shift from acid to solvent formation. Arch Microbiol in press

  • Harold FM, Pavlasova E, Baarda JR (1970) A transmembrane pH gradient in Streptococcus faecalis: origin and dissipation by proton conductors and N,N′-dicyclohexylcarbodiimide. Biochim Biophys Acta 196:235–244

    Google Scholar 

  • Hungate RE (1969) A roll tube method for cultivation. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 3b. Academic Press, London New York, pp 117–132

    Google Scholar 

  • Jarell KF, Sprott GD (1981) The transmembrane electrical potential and intracellular pH in methanogenic bacteria. Can J Microbiol 27:720–728

    Google Scholar 

  • Kell DB, Peck MW, Rodger G, Morris JG (1981) On the permeability to weak acids and bases of the cytoplasmic membrane of Clostridium pasteurianum. Biochem Biophys Res Commun 99:81–88

    Google Scholar 

  • Laanbroek HJ, Pfennig N (1981) Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and in marine sediments. Arch Microbiol 128:330–335

    Google Scholar 

  • Langworthy TA (1978) Microbial life in extreme pH values. In: Kushner DJ (ed) Microbial life in extreme environments. Academic Press, London, pp 279–314

    Google Scholar 

  • La Rivière JWM (1958) On the microbial metabolism of the tartaric acid isomers. Dissertation Univ. of Delft

  • Mitchell P (1979) Compartimentation and communication in living cell systems. Ligand conduction: A general catalytic principle in chemical osmotic and chemiosmotic reactions. Eur J Biochem 95:1–20

    Google Scholar 

  • Padan E, Zilberstein D, Rottenberg H (1976) The proton electrochemical gradient in Escherichia coli cells. Eur J Biochem 63:533–541

    Google Scholar 

  • Padan E, Zilberstein D, Schuldiner S (1981) pH-Homeostasis in bacteria. Biochim Biophys Acta 650:151–166

    Google Scholar 

  • Riebeling V, Thauer RK, Jungermann K (1975) The internal alkaline pH-gradient sensitive to uncoupler and ATPase inhibitor in growing Clostridium pasteurianum. Eur J Biochem 55:445–453

    Google Scholar 

  • Schmidt K, Liaaen-Jensen S, Schlegel HG (1963) Die Carotinoide der Thiorhodaceae. Arch Mikrobiol 46:117–126

    Google Scholar 

  • Wolin EA, Wolfe RS, Wolin MJ (1964) Viologen dye inhibition of methane formation by Methanobacillus omelianskii. J Bacteriol 87:993–998

    Google Scholar 

  • Zehnder AJB, Wuhrmann K (1976) Titanium III citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194:1165–1166

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Menzel, U., Gottschalk, G. The internal pH of Acetobacterium wieringae and Acetobacter aceti during growth and production of acetic acid. Arch. Microbiol. 143, 47–51 (1985). https://doi.org/10.1007/BF00414767

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00414767

Key words

Navigation