Archives of Microbiology

, Volume 144, Issue 2, pp 105–109 | Cite as

Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv

  • K. T. Hiriyanna
  • T. Ramakrishnan
Original Papers

Abstract

The DNA increment method, designed for measuring the increment in the amount of DNA after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (DNA) chain growth in Mycobacterium tuberculosis H37Rv growing in Youman and Karlson's medium at 37°C with a generation time of 24 h and also in relatively fast growing species like Mycobacterium smegmatis and Escherichia coli. From the results obtained, the time required for a DNA replication fork to traverse the chromosome from origin to terminus (C period) was calculated. The chain elongation rates of DNA of the three organisms was determined from the C period and the known genome sizes assuming that all these genomes have a single replication origin and bidirectional replication fork. The rate for M. tuberculosis was 3,200 nucleotides per min about 11 times slower than that of M. smegmatis and about 13–18 times slower than that of E. coli.

Key words

DNA Replication Mycobacterium tuberculosis Replication time 

Abbreviations

DNA

deoxyribonucleic acid

td

delay in initiation

OD

optical density

CAM

chloramphenicol

RIF

rifampicin

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • K. T. Hiriyanna
    • 1
    • 2
  • T. Ramakrishnan
    • 1
    • 2
  1. 1.Microbiology and Cell Biology LaboratoryIndian Institute of ScienceBangaloreIndia
  2. 2.ICMR Unit on Genetics and Cell BiologyIndian Institute of scienceBangaloreIndia
  3. 3.Department of BiologyJohns Hopkins UniversityBaltimoreUSA

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