Abstract
Glycolate can be measured in the supernatant fraction after incubation of butyrate-grown cells of Rhodospirillum rubrum either colorimetrically by the Calkins method or enzymatically using glycolate oxidase. Under optimal conditions, half-maximal excretion occurs at 11% O2 and the maximal rate is 6.9 nmol of glycolate min-1 mg protein-1 at 30°C. The pH and temperature optima are 7.6 and 30°C and light intensity is saturating in the range of 2–10×104 lux. Carbon dioxide inhibits glycolate excretion and exogenous butyrate stimulates. Glycolate excretion is maximal by butyrate-light grown cells harvested in the early stationary phase and under all conditions is proportional to the cellular content of ribulose 1,5-bisphosphate carboxylase/oxygenase.
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Abbreviations
- Bicine:
-
(N,N-bis[2-hydroxyethyl]glycine)
- RuBP:
-
d-ribulose-1,5-bisphosphate
- HPMS:
-
2-pyridylhydroxymethanesulfonate
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Storrø, I., McFadden, A. Glycolate excretion by Rhodospirillum rubrum . Arch. Microbiol. 129, 317–320 (1981). https://doi.org/10.1007/BF00414705
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DOI: https://doi.org/10.1007/BF00414705