Abstract
The thermophilic cyanobacterium Mastigocladus laminosus exhibits chromatic adaptation: In green light the production of the phycobiliprotein phycoerythrocyanin is enhanced drastically.
Phycoerythrocyanin was characterised with respect to its molecular weight, isoelectric point, absorption spectra and size of its aggregates. The two subunits of the protein were separated and characterised according to these criteria. Their chromophore contents, amino acid compositions and N-terminal amino acid sequences were also determined. The sequences were compared with those of allophycocyanin and C-phycocyanin from Mastigocladus laminosus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PEC:
-
phycoerythrocyanin
- α-PEC:
-
α-subunit of PEC
- β-PEC:
-
β-subunit of PEC
- PE:
-
phycoerythrin
- PC:
-
phycocyanin
- APC:
-
allophycocyanin
- SDS PAGE:
-
sodium dodecyl sulfate polyacrylamide gel electrophoresis
- chl a:
-
chlorophyll a
References
Bennett A, Bogorad L (1973) Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Biol 58:419–435
Binder A (1973) Isolierung und Charakterisierung des C-Phycocyanins aus der thermophilen Blaualge Mastigocladus laminosus. Diss Nr 5051 ETH Zürich
Bryant DA, Glazer AN, Eiserling FA (1976) Characterisation and structural properties of the major biliproteins of Anabaena sp. Arch Microbiol 110:61–75
Bryant DA, Guglielmi G, Tandeau de Marsac N, Castets AM, Cohen-Bazire G (1979) The structure of cyanobacterial phycobilisomes: a model. Arch Microbiol 123:113–127
Frank G, Sidler W, Widmer H, Zuber H (1978) The complete amino acid sequence of both subunits of C-phycocyanin from the cyanobacterium Mastigocladus laminosus. Hoppe-Seyler's Z Physiol Chem 359:1491–1507
Gantt E (1980) Structure and function of phycobilisomes: light harvesting pigment complexes in red and blue-green algae. Int Rev Cytol 66:45–80
Glazer AN, Bryant DA (1975) Allophycocyanin B (λMax 671, 618). Arch Microbiol 104:15–22
Glazer AN, Fang S (1973) Chromophore content of blue-green alga/phycobiliproteins. J Biol Chem 248:659–662
Gray BH, Cosner J, Gantt E (1976) Phycocyanins with absorption at 637 nm and 623 nm from Agmanellum quadruplicatum. Photochem Photobiol 24:299–302
Gysi J (1976) Isolierung und Charakterisierung der Allophycocyanine I und II und Isolierung des C-Phycocyanins aus der thermophilen Blaualge Mastigocladus laminosus. Diss Nr 5813 ETH Zürich
Gysi J, Zuber H (1974) Isolation and characterisation of allophycocyanin II from the thermophilic blue-green alga Mastigocladus laminosus. FEBS Lett 48:209–213
Gysi J, Zuber H (1976) Allophycocyanin I — a second cyanobacterial allophycocyanin? Isolation, characterisation and comparison with allophycocyanin from the same alga. FEBS Lett 58:49–54
Gysi J, Zuher H (1979) Properties of allophycocyanin II and its α-and β-subunits from the thermophilic blue-green alga Mastigocladus laminosus. Biochem J 181:577–583
Hirs CHW (1975) The oxidation of ribonuclease with performic acid. J Biol Chem 219:611–621
Koller KP, Wehrmeyer W, Mörschel E (1978) Biliprotein assembly in the disc-shaped phycolisomes of Rhodella violacea. Eur J Biochem 91:57–63
Mörschel E (1980) Structure of hemi-discoid phycobilisomes. In: Fifth international congress on photosynthesis, Halkidiki, Greece, Abstract, p 393
Nies M, Wehrmeyer W (1980) Isolation and biliprotein characterization of phycobilisomes from the thermophilic cyanobacterium Mastigocladus laminosus. Planta 150:330–337
Panyim S, Chalkley R (1969) High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys 130:337–346
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Sidler W, Isker E, Zuber H (1981) The complete amino acid sequence of both subunits of allophycocyanin, a light harvesting protein pigment complex from the cyanobacterium Mastigocladus laminosus. Hoppe-Seyler's Z Physiol Chem, in press
Sidler W, Gysi J, Isker E, Zuber H (1980) The complete primary structure of allophycocyanin from the cyanobacterium Fischerella (Mastigocladus laminosus). In: Fifth international congress on photosynthesis, Halkidiki, Greece, Abstracts, p 530
Siegelman HW, Chapman DJ, Cole WJ (1968) The bile pigments of plants. Biochem Soc Symp 28:107–120
Stainier RY (1974) The origins of photosynthesis in eukaryotes. Sym Soc Gen Microbiol 24:219–240
Tandeau de Marsac N (1977) Occurence and nature of chromatic adaptation in cyanobacteria. J Bacteriol 130:82–91
Weber K, Osborn M (1969) The reliability of molecular weight determinations by SDS-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412
Widmer H (1980) Sequenzanalytische Studien an C-Phycocyanin sowie Isolierung und Charakterisierung von Phycoerythrocyanin aus dem Cyanobakterium Mastigocladus laminosus. Diss Nr 6576 ETH Zürich
Yamanaka G, Glazer AN, Williams RC (1978) Cyanobacterial phycobilisomes. J Biol Chem 253:8303–8310
Zuber H (1978) Studies on the structure of light-harvesting pigment-protein-complexes from cyanobacteria and red algae. Ber Deutsch Bot Ges 91:459–475
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Füglistaller, P., Widmer, H., Sidler, W. et al. Isolation and characterization of phycoerythrocyanin and chromatic adaptation of the thermophilic cyanobacterium Mastigocladus laminosus . Arch. Microbiol. 129, 268–274 (1981). https://doi.org/10.1007/BF00414696
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00414696