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Der Einfluß der Kulturbedingungen auf den ATP-ADP-und AMP-Spiegel bei Rhodospirillum rubrum

The influence of cultural conditions on the ATP-, ADP- and AMP-pool of Rhodospirillum rubrum

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Summary

The ratio of adenine nucleotides in the cells of Rhodospirillum rubrum varies considerably depending upon the cultural and growth conditions.

There was no significant difference in the pool size of ATP, ADP and AMP comparing growing cells, which were cultivated aerobically in the dark, and cells, which were cultivated anaerobically in the light without substrate.

When the synthesis of ATP was inhibited or the ATP pool was lowered by high synthetic activity of the cells, the level of AMP was increased more drastically than that of ADP. This was true under anaerobic conditions in the light as well as in the dark. Under the same conditions in aerobically grown dark cultures, ADP and AMP increased to nearly the same extent.

In aerobic light cultures the relative content of ATP can be higher than in aerobic dark cultures or in anaerobic light cultures. In the former pyruvate accumulated.

With antimycin A (40 μg/ml) the ATP pool decreased in aerobic light cultures.

The specific content of adenine nucleotides per mg protein was: 5.5–6.2 nMol ATP; 1.5–3.4 nMol ADP; 1.0–1.8 nMol AMP in cells cultivated aerobically in the dark; 1.0–3.9 nMol ATP; 2.6–4.0 nMol ADP; 2.5–5.4 nMol AMP in cells cultivated anaerobically in the dark and 4.5–6.5 nMol ATP; 1.6–3.3 nMol ADP; 1.0–3.3 nMol AMP in cells cultivated anaerobically in the light.

An especially low ATP/AMP ratio was found when the culture conditions were such that the rate of synthesis of bacteriochlorophyll was higher than the growth rate.

The influence of the adenine nucleotides on the regulation of morphogenesis in Rhodospirillum rubrum has been discussed.

Zusammenfassung

Das Verhältnis der Adenin-Nucleotide in Zellen von Rhodospirillum rubrum änderte sich stark mit den Kultur-und Wachstumsbedingungen.

Aerob wachsende Dunkelkulturen und anaerobe, ruhende Lichtkulturen unterschieden sich nur geringfügig in den Pool-Größen von ATP, ADP und AMP.

Wurde die ATP-Bildung gehemmt oder fiel der ATP-Spiegel durch hohe Syntheseleistungen der Zelle, so erhöhte sich anaerob im Licht oder im Dunkeln der AMP-Spiegel viel stärker als der ADP-Spiegel, in aerober Dunkelkultur dagegen nahmen ADP und AMP gleichmäßiger zu.

In aerober Lichtkultur konnte der relative ATP-Gehalt höher sein als in aerober Dunkel-oder anaerober Lichtkultur. Es wird Pyruvat angereichert.

Antimycin A (40 μg/ml) bewirkte in aerober Lichtkultur eine Abnahme des ATP-Pools.

Der spezifische Adenin-Nucleotid-Gehalt pro Milligramm Protein betrug: 5,5–6,2 nMol ATP; 1,5–3,4 nMol ADP; 1,0–1,8 nMol AMP in aerober Dunkelkultur; 1,0–3,9 nMol ATP; 2,6–4,0 nMol ADP; 2,5–5,4 nMol AMP in anaerober Dunkelkultur und 4,5–6,5 nMol ATP; 1,6–3,3 nMol ADP; 1,0–3,3 nMol AMP in anaerober Lichtkultur.

Einen besonders niedrigen Quotienten für ATP/AMP in den Zellen wurde unter Kulturbedingungen erhalten, bei denen die Syntheserate des Bacteriochlorophylls größer als die Wachstumsrate war.

Es wird diskutiert, ob die Adenin-Nucleotide einen Einfluß auf die Regulation der Morphogenese bei R. rubrum haben.

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Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie, Institut für Biologie II der Universität Freiburg i. Br., Freiburg i. Br., Deutschland

    G. Schön

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Abkürzungen: ATP=Adenosintriphosphat, ADP=Adenosindiphosphat, AMP=Adenosinmonophosphat, BChl.=Bacteriochlorophyll, 2,4-DNP=2,4-Dinitrophenol, NADH=reduziertes Nicotinamid-Adenin-Dinucleotid, Tris=Tris-(hydroxymethyl)-aminomethan, ÄDTA=Äthylendiamintetraessigsäure, Dinatriumsalz.

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Schön, G. Der Einfluß der Kulturbedingungen auf den ATP-ADP-und AMP-Spiegel bei Rhodospirillum rubrum . Archiv. Mikrobiol. 66, 348–364 (1969). https://doi.org/10.1007/BF00414590

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