Abstract
A defined medium of low osmolarity was developed permitting growth of Rhizobium meliloti with generation times of approximately 2.8 h doubling-1. The effects of sodium, potassium, magnesium, ammonium, chloride, sulfate, phosphate, bicarbonate and acetate ions on the growth rate of R. meliloti were determined. Sodium, potassium and ammonium ions had little effect on growth at concentrations of 100 mEq or less; magnesium ion inhibited growth severely at concentrations of 50 mEq (25 mM). Of the anions, chloride and sulfate appeared to have little effect while phosphate, bicarbonate, and acetate inhibited growth at concentrations of as little as 25 mEq. The addition of proline, glutamate, or betaine to cells growing in inhibitory concentrations of NaCl did not relieve the inhibition. When grown in the presence of inhibitory levels of NaCl, the intracellular concentration of glutamate but not of proline or gamma amino butyric acid increased 5-fold.
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References
Brown AD (1976) Microbial water stress. Bacteriol Rev 40:803–846
Csonka LN (1980) The role of l-proline in response to osmotic stress in salmonella typhimurium: Selection of mutants with increased osmotolerance as strains which over-produce l-proline. In: Hollaender A (ed) Genetic engineering of osmoregulation. Plenum Press, New York London, pp 35–52
Csonka LN (1981a) The role of proline in osmoregulation in Salmonella typhimurium and Escherichia coli. In: Hollaender A (ed) Trends in the biology of fermentations for fuels and chemicals. Plenum Press, New York London, pp 533–543
Csonka LN (1981b) Proline over-production results in enhanced osmotolerance in Salmonella typhimurium. Mol Gen Genet 182:82–86
Csonka LN (1982) A third l-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength. J Bacteriol 151:1433–1443
Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Ann Rev Plant Physiol 31:149–190
Griffin DM (1981) Water and microbial stress. Adv Microb Ecol 5:137–210
Hua S-ST, Tsai VY, Lichens GM, Noma AT (1982) Accumulation of amino acids in Rhizobium sp. Strain WR1001 in response to sodium chloride salinity. App Env Microbiol 44:134–140
Koujima I, Hayashi H, Tomochika K, Okabe A, Kanemasa Y (1978) Adaptational changes in proline and water content of Staphylococcus aureus after alteration of environemental salt concentration. App Env Microbiol 35:467–470
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Makemson JC, Hastings JW (1979) Glutamate functions in osmoregulation in marine bacterium. Appl Env Microbiol 38:178–180
Meade HM, Signer ER (1977) Genetic mapping of Rhizobium meliloti Proc Natl Acad Sci USA 74:2076–2078
Measures JC (1975) Role of amino acids in osmoregulation of nonhalophilic bacteria. Nature 257:398–400
Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119:736–747
Schobert B (1977) Is there an osmotic regulatory mechanism in algae and higher plants? J Theoret Biol 68:17–27
Vincent JM (1975) A manual for practical study of root nodule bacteria. Blackwell Scientific, Oxford
Yelton MM, Yang SS, Edie SA, Lim ST (1983) Characterization of an effective salt tolerant fast growing strain of rhizobium japonicum. J Gen Microbiol 129:1537–1543
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Botsford, J.L. Osmoregulation in Rhizobium meliloti: inhibition of growth by salts. Arch Microbiol 137, 124–127 (1984). https://doi.org/10.1007/BF00414452
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DOI: https://doi.org/10.1007/BF00414452