Abstract
The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus β-lactamase III and Bacillus licheniformis penicillinase.
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Kato, C., Nakano, Y. & Horikoshi, K. The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170. Arch. Microbiol. 151, 91–94 (1989). https://doi.org/10.1007/BF00414419
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DOI: https://doi.org/10.1007/BF00414419