Summary
A method of vitreous surface whole mount preparation in rabbits is presented. Best results have been achieved with alcohol-ether fixation and PAS staining. The distribution of cells (“hyalocytes”) in the normal vitreous demonstrates large deviations with a maximal cell content in the lower half of the globe in the zonular region, a minimal cell content in the region of the equatorial retina in the upper half of the globe and an intermediate content in the posterior region. Following photocoagulation the number of cells on the vitreous surface increases considerably after the first postoperative day and decreases slowly after the 15th day to a level slightly above normal after one month. The cell rise takes place around the area of photocoagulation and in the corresponding zonular area. Morphologically 75% to 90% of the new cells as well around the area of photocoagulation as in the zonular region cannot be differentiated from normal “hyalocytes”. This and along with the finding of mitotic activity in these cells is presumptive evidence that a real proliferation of hyalocytes may take place. This is of interest in regard to the pathogenesis of postcoagulative epiretinal fibroplasia as observed in man.
Zusammenfassung
Es wird eine Methode zur Herstellung von Flachpräparaten der gesamten Glaskörperoberfläche des Kaninchens mitgeteilt, welche cytologische Untersuchungen erlaubt. Der Zellgehalt im corticalen Glaskörper unterliegt normalerweise großen Schwankungen. Höchstwerte finden sich in der unteren Hälfte des Auges im Zonulabereich, Mindestwerte aequatorial oben. Nach Lichtcoagulation kommt es zu einer deutlichen Zellvermehrung bis zum 15. Tag mit nachfolgendem allmählichem Abfall. Diese Vermehrung findet sich sowohl um die Photocoagulationsstelle als auch in der dieser Stelle benachbarten Zonularegion. Morphologisch lassen sich 75–90% der neuen Zellen von normalen corticalen Glaskörperzellen (Hyalocyten) nicht differenzieren. Die in dieser Zellpopulation beobachteten vermehrten Mitosen beweisen, daß eine echte Proliferation von Hyalocyten stattfindet. Dies erscheint für das Verständnis der beim Menschen auftretenden postcoagulativen epiretinalen Fibroplasie wichtig.
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This investigation was conducted under the Fight For Sight Post-Doctoral Research Fellowship from Fight For Sight, Inc., New York City, No. F-209 and was supported in part by NIH Grant No. NB 10789 and B 621.
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Gloor, B.P. Cellular proliferation on the vitreous surface after photocoagulation. Albrecht von Graefes Arch. Klin. Ophthalmol. 178, 99–113 (1969). https://doi.org/10.1007/BF00414375
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DOI: https://doi.org/10.1007/BF00414375